Ag85A和GM—CSF DNA疫苗对小鼠原位膀胱癌细胞免疫功能的影响。

Effect of Ag85A Combined with GM-CSF DNA Vaccine on the Cellular Immune Function of Mice Bearing Orthotopic Bladder Cancer

【目的】探讨联合应用Ag85A与粒细胞一巨噬细胞集落刺激因子（GM—CSF）DNA疫苗对原位膀胱癌小鼠的细胞免疫的影响。【方法】以N-甲基亚硝基脲（MNU）灌注小鼠膀胱，建立小鼠原位膀胱癌模型。50只荷瘤小鼠随机分为5组：生理盐水组、空载体组、A。g85A组、联合应用组和BCG组。模型确定后d，、d。t天和dzt各肌注1次，末次注射后d，检测各组小鼠脾脏的cD4’和cD8’T细胞亚群数量及cD4。。／CD8’比值和血清7一干扰素（IFN一7）。【结果】单独应用Ag85A和联合应用Ag85A与GM-CSFDNA疫苗均能够升高CD4^＋和CD8^＋T细胞的百分比及CD4^＋和CD8^＋T的比值，提高血清IFN-7，差异具有统计学意义（P〈0．05）。联合应用的效果明显优于单独应用，差异具有统计学意义（P〈0．05），但尚不及单纯应用BCG的免疫治疗效果。【结论】联合应用Ag85A与GM—CSF DNA疫苗能够提高荷瘤小鼠的细胞免疫功能，其效果明显优于Ag85 ADNA疫苗。

[Objective] To explore the effect of Ag85A combined with GM-CSF DNA vaccine on the cellular immuni- ty in mice bearing orthotopic bladder cancer. [Methods] The mouse model of orthotopic bladder cancer was established with N-methyl-nitroso-urea（MNU） infused into mouse bladder. Fifty tumor-bearing mice were randomly divided randomly into normal saline（NS） group, pcDNA3.1 group, Ag85A group, Ag85A combined with GM-CSF group and BCG group. After the establishment of the models, the intramuscular injection was taken one time at 7th, 14th and 21th. The number of CD4^＋ and CD8^＋ T cell subsets, the ratio of CD4^＋ / CD8^＋ and serum IFN-7 in mouse spleen of each group were detected 7 days after the last injection. [Results] Both single Ag85A and Ag85A combined with GM-CSF vaccine could elevated the percentage of CD4^＋ and CD8^＋ T cell subsets, the ratio of CD4^＋/CD8^＋ and serum IFN-7 level, and there was significant difference（ P d0.05）. The immunotherapy effect of Ag85A combined with GM-CSF vaccine was much better than that of Ag85A vaccine alone, and there was significant difference between them（ P d0.05）. But the immunotherapy effect of Ag85A combined with GM-CSF vaccine was still inferior to BCG vaccine. [Conclusion]Ag85A combined with GM-CSF vaccine can increase the cellular immunity in bearing bladder tumor-bearing mice, and its effect is much better than that of Au85A DNA vaccine alone.