摘要
目的检测DNA结合抑制物2(inhibitor of DNA binding 2,Id2)在大鼠脑缺血再灌注海马组织神经干细胞中的表达及bFGF的干预作用。探讨影响神经干细胞增殖分化的分子调控机制。方法采用大脑中动脉栓塞(MCAO)制作大鼠脑缺血再灌注模型。用免疫组织化学法检测海马神经干细胞BrdU、Id2的表达及bFGF的干预作用。结果随着脑缺血再灌注第3天缺血海马神经元BrdU阳性细胞明显增多,第7天达高峰,Id2反应产物脑缺血再灌注第3天较正常组增多,随缺血再灌注时间的延长逐渐增多,第7天表达最强,以后表达逐渐减少。注射bFGF后BrdU、Id2的表达明显增加。结论 bFGF促进神经干细胞的增殖及缺血脑组织Id2的表达,提示bFGF促进神经干细胞增殖作用可能由Id2信号介导。
Objective To investigate the effects of basic fibroblast growth factor（bFGF） on Id2 expression and hippocampal neurogenesis in a rat model of cerebral ischemia / reperfusion,explore the related molecular mechanism.Methods The model of transient focal ischemia was made by occlusion of the middle cerebral artery,the expression of BrdU and Id2 and in hippocampus was detected with immunohistochemical method analyzed by microimage system.Results Neural stem cells were activated after cerebral ischemia.After three days,BrdU positive cells in hippocampus were obviously increased.To the seventh day,BrdU positive cells were more than those at any time.The expression of Id2 had similar trend with the expression of BrdU.Following bFGF injection,the expression of hippocampal Id2 had apparently increased in each group.Conclusions bFGF promotes the expression of Id2 in the hippocampus tissue.These findings indicate that bFGF promotion of neural stem cell proliferation may be mediated by Id2 signaling pathway.
出处
《解剖学研究》
CAS
2011年第1期9-12,共4页
Anatomy Research
基金
辽宁省教育厅科学研究计划资助项目(L2010546)
