MOG1-125诱发的实验性自身免疫性脑脊髓炎大鼠模型

SD rat models of experimental autoimmune encephalomyelitis induced by MOG1-125

目的建立髓鞘少突胶质细胞糖蛋白多肽1-125(MOG1-125)诱发的实验性自身免疫性脑脊髓炎(EAE)大鼠模型。方法采用大鼠MOG1-125和福氏完全佐剂作为抗原。在第1天和第15天分别进行两次皮内注射抗原,免疫SD大鼠建立EAE的动物模型,观察其临床症状,取脊髓组织进行冰冻切片、半薄切片和超薄切片,光镜和电镜观察脊髓组织学改变。结果 SD大鼠在两次免疫后8～10 d发病,其发病率为15%。在快蓝染色和甲苯胺蓝染色后,发现脊髓白质存在广泛的脱髓鞘浅染区。通过透射电镜观察进一步证实脊髓白质存在脱髓鞘现象。结论采用大鼠MOG1-125和完全弗氏佐剂作为抗原对SD大鼠进行两次免疫,能够成功诱发出EAE模型。

Objective To establish rat models of experimental autoimmune encephalomyelitis（EAE） induced by peptide myelin oligodendrocyte glycoprotein 1-125（MOG1-125）.Methods SD rats were immunized twice,at days 1 and 15,by intradermal injection at the base of the tail with a 100 μL cocktail solution containing;50 μL of 50 μg recombinant rat MOG corresponding to the N-terminal sequence of rat MOG（amino acids 1-125） in saline（1∶1）,and 50 μl of CFA（complete Freund＇s adjuvant from Chondrex Inc.）,200 μg heat-inactivated mycobaterium tuberculosis（strain H 37 RA）.Both the clinical symptoms and histopathologic changes of the spinal cord were observed with the aid of light and electron microscope.Results The animals developed the typical symptoms of EAE on 8th to 10th day after the second immunization,which showed the incidence of 15%.A mass of demyelinated white matter were observed through Luxol fast blue staining and toluidine blue staining respectively with an optical microscope.Furthermore,demyelination was also found in the white matter of spinal cord by electron microscopy.Conclusion EAE model of SD rats is successfully induced by intradermal injection with a cocktail solution containing of MOG1-125 and CFA.