匹罗卡品诱发癫痫持续状态大鼠模型的改良和鉴定

Improvement and evaluation of model of pilocapine induced status epilepsticus

目的对匹罗卡品诱发的癫痫持续状态大鼠模型进行改良和鉴定。方法 SD大鼠(6～8周,雌性)注射氯化锂、丁溴东莨菪碱、匹罗卡品诱发癫痫持续状态,分别在急性期和慢性自发癫痫形成期接受脑电监测和海马病理学检查。结果行为学上急性期匹罗卡品组(PISE)出现Racine分级的IV级或以上发作,慢性自发癫痫形成期每周可见自发癫痫发作。脑电图急性期表现为低于6 Hz的慢活动逐渐增多,随后出现爆发长程的棘活动;慢性自发癫痫形成期可见较多散在低于6 Hz的慢活动和棘波发放。尼氏染色PISE组急性期CA1区仅见少量坏死神经元;慢性自发癫痫形成期见锥体细胞排列紊乱,部分神经元缺失导致锥体细胞层中断。结论本研究改良了匹罗卡品诱发癫痫持续状态模型,其行为学表现、电生理特征和病理学变化类似于人类获得性颞叶内侧癫痫。

Objective To the improvements and evaluation of the model of pilocarpine-induced status epilepticus（PISE）.Methods The Sprague-Dawley rats（6-8 weeks,female） were injected lithium chloride,scopolamine methyl bromide and pilocarpine.The rats were recorded of EEG until they were sacrificed for the observation of hippocampus pathological alterations.Results Status epilepticus of Racine IV or V was induced by the treatment of pilocarpine in PISE group.Spontaneous epilepstic seizures could be observed in the chronic stage.EEG showed bursts of spikes and waves in acute SE phase,while EEG monitoring showed epileptic discharges during the chronic phase in the inter-ictal phase.Nissl staining showed the hippocampal neuronal loss and the decrease of Nissl bodies.Conclusion The improved model of PISE is similar to acquired mesial temporal lobe epilepsy in behavior,electrophysiology and pathology.