摘要
目的研究糖尿病大鼠(OLETF鼠)和罗格列酮治疗后,睾丸的组织病理变化及各级生精细胞中Bcl-xL(Bcl-x long)表达的改变。方法 OLETF雄性大鼠20只,定期口服葡萄糖耐量试验(OGTT)监测血糖。喂养至30周时,共有成模OLETF鼠12只,随机分为罗格列酮组和模型组(每组6只),LETO鼠8只作为对照组,各组均给药12周。处死大鼠,测定睾丸重量,HE染色光镜观察睾丸组织病理变化,并运用免疫组化方法对各级生精细胞Bcl-xL蛋白表达情况进行检测。结果与对照组相比,模型组和罗格列酮组有明显的病理变化。睾丸重量、精原细胞和间质细胞计数、睾丸生精细胞Bcl-xL蛋白阳性表达强度,模型组比对照组降低(P<0.05),而罗格列酮组与模型组之间差异并无统计学意义(P>0.05)。结论糖尿病可能通过细胞凋亡机制影响生精过程,而罗格列酮对糖尿病生精障碍并无改善作用。
Objective To study the testis,change of pathological and Bcl-xL in diabetes mellitus rats,and influence of rosiglitazone.Methods Blood glucose was determined by OGTT for 20 male OLETF rats.After 30 weeks,12 T2DM OLETF rats were randomly divided into rosiglitazone group and model group（n = 6 per group）.Eight male LETO rats were used as control group.All groups were administration drugs or water via intragastrically for 12 weeks.After the rats were sacrificed,weigh the testis,Observe their pathological change by using optical microscope and detect the expression of Bcl-xL in spermatogenic cells through making use of immunohistochemistry.Results Compared with the control group,model group and rosiglitazone group have significant pathological changes,had lower in the weight of testis,the count of spermatogoniums and interstitial cells,the positive expression of Bcl-xL（P0.05）.Rosiglitazone and model had nonstatistical differences between groups.Conclusion Diabetes mellitus may influence the spermatogenic process through apoptosis mechanisms,rosiglitazone hasn＇t improve the spermatogenic process of diabetes mellitus.
出处
《解剖学研究》
CAS
2011年第2期138-141,共4页
Anatomy Research
