血清HSP27在原发性肝细胞癌早期诊断中的应用

The application of serum HSP27 on early diagnosis of hepatocellular carcinoma

目的探讨热休克蛋白27(HSP27)在原发性肝细胞癌(HCC)早期诊断中的应用。方法采用酶联免疫吸附试验(ELISA),检测正常人、乙肝病毒携带者和HCC患者血清中HSP27的含量,并结合化学发光免疫分析法进行血清甲胎蛋白(AFP)的平行检测。结果 HCC患者平均HSP27水平明显高于正常对照组和HBV携带者。其中大/小肝癌组、或AFP阴性/AFP阳性HCC组与正常对照组、慢性HBV感染组及急性HBV感染组比较,差异均有统计学意义。所有肝癌组与AFP阳性的HBV感染组相比差异也有统计学意义。并且,当HSP27与经典标志物AFP相结合检测HCC时,诊断的敏感性、特异性、阳性预测值均大大提高。结论 HSP27可以作为HCC的早期血清学诊断的生物标志物。HSP27和AFP平行试验可提高敏感度,减少误诊率,有利于临床诊断工作。

Objective To explore the application of heat shock protein 27 （HSP27） on early diagnosis of Hepatocellular carcinoma （HCC）. Methods Serum HSP27 levels of HCC patients, HBV carrieries and healthy controls were detected by means of ELISA, which combined with Serum AFP parallel detection of the same population using chemiluminescence immunoassay （CIA）. Results The average HSP27 level of HCC patients was significantly higher than that of HBV carriers or healthy controls. Among them, the average HSP27 level of large/small HCC group or AFP negative/AFP positive HCC group showed significant difference compared with healthy control group, chronic HBV infected group and acute HBV infected group. Moreover, The the average HSP27 level also showed significant diffcience between HCC group and AFP positive HBV infected group. Conclusion The parallel detection of HSP27 and AFP may useful to clinical diagnosis by increasing the sensitivity and reducing the misdiagnosis rate.