甲异靛对人乳腺癌MCF-7细胞增殖和凋亡影响的实验研究

Effect of meisoindigoon the proliferation and apoptosis in human breast carcinoma MCF-7 cells

目的:研究甲异靛对人乳腺癌MCF-7细胞增殖抑制和诱导凋亡作用。方法:采用MTT法检测甲异靛对MCF-7乳腺癌细胞的增殖抑制;流式细胞仪测定细胞凋亡率;光学显微镜观察细胞形态的变化;westernblot法测定caspase-3、PARP及bcl-2表达。结果:甲异靛能明显抑制MCF-7乳腺癌细胞增殖。甲异靛诱导MCF-7细胞凋亡,呈现典型细胞凋亡的形态变化,并呈时间和剂量依赖性,凋亡率最高可达(68.40±4.87)%,在细胞凋亡过程中出现PARP分子断裂和bcl-2下调,未检测到caspase-3。结论:甲异靛能诱导MCF-7乳腺癌细胞凋亡抑制细胞增殖,其发生机制可能与下调bcl-2有关。

Objective：To explore the effects of meisoindigo on cell growth inhibition and apoptosis in MCF-7 human breast cancer cell lines.Methods：The proliferation inhibitory was evaluated by MTT test.Cell morphological alteration was observed by using light microscopy.The cell apoptosis was detected by flow cytometry.The expression levels of caspase-3,PARP and bcl-2 were determined by western blot.Results： Meisoindigo could markedly inhibit MCF-7 cells proliferation,the typical morphological changes were observed in MCF-7 cells after treated by meisoindigo under light microcopy,meisoindigo induced apoptosis in MCF-7cells in a concentration-and time-dependent manner,the highest apoptosis rate was（68.40±4.87）%.The level of bcl-2 in MCF-7cells was decreased and full-length PARP and its cleavage product were indicated after meisoindigo treatment,however,caspase-3 was not found in MCF-7 cells.Conclusion： Meisoindigo can inhibit proliferation and induce apoptosis of MCF-7 breast cancer cells.The mechanism may be related with down-regulating bcl-2,without caspase-3.