鱼藤酮和MPTP慢性帕金森病小鼠黑质共同差异蛋白研究

Common differentially expressed proteins in substantia nigra of mouse models with chronic Parkinson's disease induced by rotenone and MPTP

目的通过比较鱼藤酮和1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)两种慢性帕金森病(Parkinson’s disease,PD)模型小鼠与对照组小鼠黑质蛋白表达谱,找出两种PD模型小鼠共同的差异蛋白,探索筛选出PD的疾病特异性蛋白。方法构建鱼藤酮和MPTP慢性PD小鼠模型组以及其各自的对照组1和对照组2,并进行行为学评价以及黑质酪氨酸羟化酶(tyrosine hydroxylase,TH)免疫组化。分别挖取黑质,提取总蛋白,进行双向凝胶电泳。运用PDQuest 8.0图像分析软件对鱼藤酮组与对照组1、MPTP与对照组2的电泳图谱分别进行分析,找出各自的差异表达蛋白。差异蛋白酶切后,MALDI-TOF-MS进行质谱鉴定。最后将所得到的肽质量指纹图(PMF)进行数据库搜索以及生物信息学分析。结果鱼藤酮组与对照组1、MPTP组与对照组2小鼠间行为学差异具有统计学意义(P<0.05),且黑质TH阳性细胞计数差异也具有统计学意义(P<0.05)。鱼藤酮组与对照组1比较发现有22个差异蛋白,MPTP组与对照组2比较发现有28个差异蛋白,其中有7个为共同的差异蛋白。我们成功鉴定其中3个共同的差异蛋白:PPP2R1A、吡哆醛激酶、peroxiredoxin-2。结论鉴定出3个在PD蛋白质组学中少见报道的蛋白。这些蛋白水平的上调或下调可能与PD的发病密切相关,可作为PD的疾病特异性蛋白。

Objective To search for the common differentially expressed proteins in the substantia nigra of mouse models with Parkinson＇s disease（PD） induced by rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）,and to screen out the disease-specific proteins（DSPs） of PD.Methods Forty-eight mice were randomly divided into a rotenone group and its control group 1,as well as an MPTP group and its control group 2.In the rotenone group and MPTP group,rotenone and MPTP were given separately by injection to construct mouse models with chronic PD.Behavioral evaluation of the mice in the four groups was performed,and immunohistochemistry was carried out to detect the tyrosine hydroxylase（TH）-positive cells in the substantia nigra.Proteins were extracted from the substantia nigra and subjected to two-dimensional gel electrophoresis.Electrophoretogram analysis was performed using PDQuest 8.0 to find out the differentially expressed proteins between the rotenone group and control group 1 and between the MPTP group and control group 2.The differentially expressed proteins,after digestion,were subjected to MALDI-TOF-MS to obtain their peptide mass fingerprints for searching in a database and biological information analysis.Results There were significant behavioral differences between the rotenone group and control group 1 and between the MPTP group and control group 2（P0.05）.The numbers of TH-positive cells also showed significant differences between the rotenone group and control group 1 and between the MPTP group and control group 2（P0.05）.Twenty-two differentially expressed proteins were found between the rotenone group and control group 1,and twenty-eight were found between the MPTP group and control group 2,with seven common differentially expressed proteins.Three of the seven common differentially expressed proteins,PPP2R1A,pyridoxal kinase and peroxiredoxin-2,were identified in the database.Conclusion Three common differentially expressed proteins,not reported in the previous proteomic research on PD,are identified.Their up-regulated or down-regulated expression may be intimately related to the pathogenesis of PD.The three common differentially expressed proteins may be DSPs of PD.