鲤鱼血清谷丙转氨酶活性和活性抑制的测定

THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS TNHIBITION ON SGPT OF CARP

鲤鱼在实验室条件下（１８ ±３ ℃） 驯化７ 天，其血清谷丙转氨酶（ＳＧＰＴ） 的活性在显著水平为Ｐ≤０ ．０５ 时，不同组鲤鱼（２０ ～３０ ｃｍ） 无显著差异．离体实验条件下，分析了单甲脒（ＤＭＡ） ，对硝基苯酚ｐ － ＮＰｈ 和邻硝基苯酚ｏ － ＮＰｈ 三种有机化合物对鲤鱼谷丙转氨酶均有明显的抑制作用，鲤鱼的半抑制浓度值分别为１０５ ｍｇ／Ｌ，７ ．１４ ｍｇ／Ｌ，２２ ．５ ｍｇ／Ｌ．鲤鱼血清谷丙转氨酶（ＳＧＰＴ） 可作为一项指标用于生态毒理学研究．

Under normal condition,It was proved that difference of SGPT(Serum glutamic pyruvic transaminase)from different groups of carp(23～27 cm)acclimated under 18±3℃ for 7 days was not significant (p≤0.05) in the laboralory.Then tests of the effects of DMA,DMA、 p -NPh and o -NPhon SGPT of carp in vitro were carried out.The results showed that DMA、 p -NPh ang o -NPh Caused fairly strong inhibition to SGPT of carp.The concentration required to effect 50% inhibition of DMA, p -NPh and o -NPhto SGPT of carp were 105 mg/L, 7.14mg/L and 22.55mg/L, respectively.It was proposed that effects of toxicant on SGPT of carp could be used as an index of toxicological assessment.