补肾调冲方对去卵巢大鼠性激素水平及下丘脑雌激素受体α、雌激素受体β mRNA表达的影响

Effects of the Bushen Tiaochong decoction on the levels of serum sex hormone and the expression levels of estrogen receptors α,β mRNA in the hypothalamus in ovariectomized rats

[目的]探讨补肾调冲方对去卵巢大鼠血清性激素水平及下丘脑雌激素受体α(ERα)、雌激素受体β(ERβ)mRNA表达的影响。[方法]以假手术组为对照,将去卵巢大鼠随机分为去卵巢空白组,补肾调冲方高、中、低剂量组,已烯雌酚组。采用放射免疫分析法测定血清雌二醇(E2)、促卵泡生成激素(FSH)、促黄体生成激素(LH)水平,采用逆转录-聚合酶联反应(RT-PCR)检测下丘脑ERα、ERβmRNA表达。[结果]去卵巢大鼠血清FSH、LH水平升高,E2及下丘脑ERα、ERβmRNA水平降低,补肾调冲方大鼠血清E2有升高趋势,FSH有降低趋势,补肾调冲方能降低去卵巢大鼠血清LH水平,升高下丘脑ERα、βmRNA的表达。[结论]补肾调冲方在改善去卵巢大鼠性激素水平同时,可能通过影响ERα、ERβmRNA的表达,提高ER的生物效应,从而降低更年期综合征的发生。

[Objective] To investigate the effects of the Bushen Tiaochong decoction on the levels of serum sex hormone and the expression levels of estrogen receptors α,β（ERα,ERβ） mRNA in the hypothalamus in ovariectomized rats.[Methods] Sham operation group was considered as control,castrated rats were divided into four groups： ovariectomized blank group,Bushen Tiaochong decoction high dose group,moderate dose,low dose group,Diethylstilbestrol group.The levels of serum E2,FSH,LH were determraed by radioimmunoassay method,and the RT-PCR method was used to detect the expression levels of ERα,ERβ mRNA in the hypothalamus.[Results] The levels of serum FSH,LH in ovariectomized rats was higher as well as the levels of estrogenic and ERα,ERβ mRNA in the hypothalamus was lower.Bushen Tiaochong decoction group on the levels of serum estrogenic in ovariectomized rats has a rising trend and the levels of FSH has a declining trend.Bushen Tiaochong decoction reduced the levels of serum LH and increased the expression levels of α,βmRNA in the hypothalamus.[Conclusion] Bushen Tiaochong decoction improve the levels of sex hormones in ovariectomized rats and through increasing the expression levels of ERα,ERβ mRNA to improve ER biological effects and reduce the occurrence of menopausal syndrome.