食用白酒对实验性肝纤维化TGF-β_1的影响

Study on Effect of Maotai Liquor on Expression of TGF-β_1 in Hepatic Fibrosis of Experimental Rat

目的:观察实验性大鼠肝纤维化转化生长因子-β1(TGF-β1)的表达,探讨食用白酒(贵州茅台酒)对肝纤维化的影响。方法:雄性Wistar大鼠79只随机分为:正常对照组(n=9)、茅台酒四氯化碳组(n=12)、乙醇四氯化碳组(n=13)、茅台酒组(n=14)、乙醇组(n=16)、四氯化碳造模组(n=15)。实验第4周和第8周末分批处死动物40、37只,取血和肝脏,分别行血清肝功能、肝纤维化血清学指标检测、HE染色评估肝纤维化程度、免疫组化法检测肝组织中转化生长因子-β1(TGF-β1)水平。结果:乙醇四氯化碳组和茅台酒四氯化碳组间层粘连蛋白(LN)差异有统计学意义(P<0.05)。在第8周末,茅台酒四氯化碳组的Ⅲ型前胶原(PCⅢ)与四氯化碳造模组差异有统计学意义(P<0.05)。对血清LN、PCⅢ、肝脏TGF-β1表达的影响,茅台酒组和乙醇组相比较,差异有统计学意义(P<0.05)。结论:茅台酒没有显著增加TGF-β1的表达,对肝纤维化的发展有一定的抑制作用,同乙醇相比,茅台酒可以延缓四氯化碳造成的纤维化损伤。

Objective:To investigate the effect of Kweichow Maotai liquor(Maotai) on the expression of transforming growth factor beta-1(TGF-β1) in the hepatic fibrosis experimental rat.Methods:Seventy-nine male Wistar rats were randomly divided into six groups: normal group,Maotai liquor and carbon tetrachloride group,alcohol and carbon tetrachloride group,Maotai liquor group,alcohol group and carbon tetrachloride group.At the end of the fourth and eighth week,liver tissues were taken for morphologica analysis by HE and the stage of hepatic fibrosis was observed under light microscope.Blood samples were collected to measure serum transaminases(AST & ALT) and ALB.Serum pro-collagenⅢN-terminal peptide(PⅢNP) and laminin(LN) were measured by the radio-immunity technique.TGF-β1 was determined with immunohistochemistry assay.Results: The differences of serum LN between Maotai liquor and carbon tetrachloride group and alcohol and carbon tetrachloride group were significant(P<0.05).At the end of the eighth week,compared with carbon tetrachloride group,PⅢNP was markedly decreased in serum of the Maotai liquor and carbon tetrachloride group(P<0.05).The serum LN,PⅢNP and the expression of TGF-β1 and Smad4 were significant(P<0.05) between Maotai liquor group and alcohol group.Conclusion:The expression of TGF-β1 in the experimental rats does not significantly grow from the drinking of Maotai liquor,which shows that Maotai liquor can inhibit the formation and development of liver fibrosis.Compared with alcohol,Maotai liquor has the inhibitory effect on hepatic fibrosis induced by CCl4.