高效液相色谱法测定复方维E乳膏中维生素E的含量

Determination of Vitamin E in Compound Vitamin E Cream by HPLC

目的建立高效液相色谱法测定复方维E乳膏中维生素E的含量。方法采用高效液相色谱法。色谱柱为Sh im-pack VP-ODSC18,流动相为甲醇,流速为1.0 m l/m in,检测波长为284 nm,进样量为20μl。结果维生素E在0.068~0.340 mg/m l浓度范围内线性关系良好（r=1.000 0）,平均回收率为102.3%,RSD为0.69%。结论本法简便、快速、准确,回收率好,可用于复方维生素E乳膏的质量控制。

Objective To establish a method for the content determination of vitamin E in compound vitamin E cream by HPLC.Methods Shim-pack VP-ODS C18 column was used,and the mobile phase consisted of methanol at the flow rate of 1.0 ml/min.The detection wavelength was set at 284 nm.The column temperature was set at room temperature.Injection volume was 20 μl.Results The linear range of vitamin E was 0.068~0.340 mg/ml（r=1.000 0）,with an average recovery of 102.3%,RSD=0.69%.Conclusion The method is simple,sensitive,and accurate for the quality control of compound vitamin E cream.