缺氧对食管癌细胞缺氧诱导因子-1α及己糖激酶表达的影响

Influence of hypoxia on hypoxia-inducible factor-1α and Hexokinase-Ⅱ in esophageal carcinoma

目的探讨不同缺氧程度对食管癌细胞株TE1中缺氧诱导因子-1α(Hypoxia-inducible factor-1α,HIF-1α)及糖酵解关键酶表达的影响。方法 TE1细胞分别在正常氧分压和缺氧条件下培养,缺氧时间设定为6、12、24及48h,使用Western blot方法检测缺氧培养不同时间后细胞中HIF-1α及糖酵解关键酶己糖激酶II(Hexokinase-II,HK-II)的蛋白水平表达变化。结果常氧及缺氧条件下TE1细胞中HIF-1α及HK-II均有表达,缺氧后表达均较常氧培养时明显增强,且随缺氧时间不同而呈先增多后减少的动态变化。结论低氧能够增加食管癌细胞中HIF-1α及HK-II表达而促进糖酵解进程,联合抑制HIF-1α和糖酵解酶可能成为治疗食管癌的潜在的靶点。

Objective To study the influence of hypoxia on hypoxia-inducible factor-1α（HIF-1α） and glycolysis associated protein in esophageal carcinoma TE1.Methods Esophageal cancer TE1 were incubated under normoxic and hypoxic conditions for different time（6,12,24,48 h）.Expressions of HIF-1α protein and the associated enzyme in glycolysis（HK-II） were detected by Western blot.Results HIF-1α and HK-II protein were both expressed in normoxic conditions.The expression of HIF-1α and HK-II increased after incubated in hypoxia environment,both reached perk at 12 h（P0.05）.Conclusion Hypoxia condition can up-regulate the expression of HIF-1α and HK-II.Joint suppression of HIF-1α and key enzymes in glycolysis could become the potential targets for treatment of esophageal cancer.