免疫法制作大鼠非细菌性前列腺炎模型的量效关系研究

Establishment of a nonbacterial prostatitis model in rats using the autoimmune method: A dose-effect study

目的:探讨用不同剂量前列腺蛋白提纯液辅以弗氏完全佐剂,建立慢性非细菌性前列腺炎动物模型并探讨模型成功与剂量的关系。方法:将30只Wistar大鼠随机分为A～E共5组,每组6只,其中A组为对照组,B～E组为实验组,在第0、30天,多点皮内注射浓度为20(B组)、40(C组)、60(D组)、80 mg/ml(E组)的前列腺蛋白提纯液及弗氏完全佐剂1∶1混悬液1.0 ml,同时腹腔注射百白破疫苗0.5 ml,第45天观察大鼠前列腺组织的大体及病理形态学。结果:各实验组表现出不同程度的慢性炎症,有不同程度的淋巴细胞浸润和间质增生,且存在量效关系。其中C、D组动物前列腺病变范围、淋巴浸润程度、间质增生程度与A组比较,差异均有显著性(P均<0.05),E组上述变化更明显。结论:30 d内两次多点皮内注射浓度40～60 mg/ml的大鼠前列腺蛋白提纯液与弗氏完全佐剂的1∶1混悬液1.0 ml,同时腹腔注射百白破疫苗0.5 ml,即可成功地建立大鼠慢性非细菌性前列腺炎模型。

Objective： To establish an animal model of chronic nonbacterial prostatitis （CNP） using different doses of purified prostate protein with Freund＇s complete adjuvant （ FCA）, and to investigate the relationship of the doses with the success of the model construction. Methods： Thirty male Wistar rats were divided into a control （A） and 4 experimental groups （B, C, D and E） of equal number. The latter 4 groups were given multi-loci intracutaneous injection of 1.0 ml of a 1 ： 1 mixture of purified prostate protein at 20, 40, 60 and 80 mg/ml with Freund＇s complete adjuvant （FCA）, and meanwhile intraperitoneally injected with 0. 5 ml of pertussis-diphtheria-tetanus vaccine at 0 and 30 days. On the 45th day, the rats were sacrificed for observation of the pathomorphological changes in the prostate glands with the naked eyes and microscope. Results： Different degrees of chronic inflammation were observed with different degrees of lymphocyte infiltration and interstitial hyperplasia in the experimental rats. More obvious changes were found in Groups C and D than in A, and even more significant in Group E （P 〈 0.05）. Conclusion ： The rat model of CNP can be success- fully established by multi-loci intracutaneous injection of 1.0 ml of a 1 ： 1 mixture of purified prostate protein at 40 - 60 mg/ml with FCA, and simultaneously intraperitoneal injection of 0.5 ml of pertussis-diphtheria-tetanus vaccine twice within 30 days.