摘要
目的探讨白细胞介素1(interleukin-1,IL-1)基因型与阿尔茨海默病(Alzheimer’Sdisease,AD)的关系。方法应用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)技术,对84例AD和139例对照组标本进行IL-lA(-889)和IL-1B(+3953)基因型分析。结果AD组中IL-1A(-889)C/C、C/T和T/T基因型的频率分别为72.6%、23.8%和3.6%,对照组中C/C、C/T和T/T基因型的频率分别为84.2%、14.4%和1.4%,AD组和对照组IL-1A(-889)各基因型的频率差异无统计学意义(X^2=4.53,P〉0.05),但AD组IL-1A(-889)T等位基因的分布频率明显高于对照组,频率分别为15.5%(AD组)和8.6%(对照组)(X^2=4.93,P〈0.05)。AD组中IL-1B(+3953)C/T基因型和T等位基因的频率分别为16.7%和8.3%,均显著高于对照组6.5%和3.2%(X^2=5.88、5.56,均P〈0.05)。结论IL-1基因型与AD的发生有关,可能是AD的重要危险因素。
Objective To explore the association of interleukin (IL)-I genotypes with Alzheimer's disease (AD). Methods Using polymerase chain reaction and restriction fragment length polymorphism, the IL-1A ( 889) and IL-1B (+3953) genotypes in 84 cases of AD and 139 controls were detected and analyzed. Results The frequencies of IL-1A(- 889) C/C, C/T and T/ T genotypes were 72.6% and 84.2%, 23.8% and 14.4%, 3.6% and 1.4% in AD cases and controls respectively. The genotypes frequencies of IL-1A (-889) C/C, C/T and T/T in AD cases were similar to that of controls (X^2=4.53, P〉0.05), but the frequencies of IL-1A (-889) T allele were significantly higher in AD cases than in controls (15.5% vs. 8.6%, X^2==4.93, P〈0.05). The frequencies of IL-1B (+3953) C/T genotypes and T allele were also significantly higher in AD cases thanin controls (16.7% vs. 6.5%, 8.3% vs. 3.2%, X^2= =5.88,5.56, both P〈0.05). Conclusions IL-1 genotypes are associated with AD. IL-1 genotypes may play an important role in the development of AD.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2011年第7期533-536,共4页
Chinese Journal of Geriatrics
基金
首都医学发展科研基金(2009-3017)