白细胞介素1基因型与阿尔茨海默病的相关性研究

Association study of interleukin-1 genotypes with Alzheimer＇s disease

目的探讨白细胞介素1（interleukin-1，IL-1）基因型与阿尔茨海默病（Alzheimer’Sdisease，AD）的关系。方法应用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）技术，对84例AD和139例对照组标本进行IL-lA（-889）和IL-1B（＋3953）基因型分析。结果AD组中IL-1A（-889）C／C、C／T和T／T基因型的频率分别为72．6％、23．8％和3．6％，对照组中C／C、C／T和T／T基因型的频率分别为84．2％、14．4％和1．4％，AD组和对照组IL-1A（-889）各基因型的频率差异无统计学意义（X^2=4．53，P〉0．05），但AD组IL-1A（-889）T等位基因的分布频率明显高于对照组，频率分别为15．5％（AD组）和8．6％（对照组）（X^2=4．93，P〈0．05）。AD组中IL-1B（＋3953）C／T基因型和T等位基因的频率分别为16．7％和8．3％，均显著高于对照组6．5％和3．2％（X^2=5．88、5．56，均P〈0．05）。结论IL-1基因型与AD的发生有关，可能是AD的重要危险因素。

Objective To explore the association of interleukin （IL）-I genotypes with Alzheimer＇s disease （AD）. Methods Using polymerase chain reaction and restriction fragment length polymorphism, the IL-1A （ 889） and IL-1B （＋3953） genotypes in 84 cases of AD and 139 controls were detected and analyzed. Results The frequencies of IL-1A（- 889） C/C, C/T and T/ T genotypes were 72.6% and 84.2%, 23.8% and 14.4%, 3.6% and 1.4% in AD cases and controls respectively. The genotypes frequencies of IL-1A （-889） C/C, C/T and T/T in AD cases were similar to that of controls （X^2=4.53, P〉0.05）, but the frequencies of IL-1A （-889） T allele were significantly higher in AD cases than in controls （15.5% vs. 8.6%, X^2==4.93, P〈0.05）. The frequencies of IL-1B （＋3953） C/T genotypes and T allele were also significantly higher in AD cases thanin controls （16.7% vs. 6.5%, 8.3% vs. 3.2%, X^2= =5.88,5.56, both P〈0.05）. Conclusions IL-1 genotypes are associated with AD. IL-1 genotypes may play an important role in the development of AD.