灵芝多糖对α-萘异硫氰酸酯致大鼠肝损伤的保护作用

Protective effect of Ganoderma lucidum polysaccharides on liver injury in jaundice model of rat induced by ANIT

目的:观察灵芝多糖(GLP)对α-萘异硫氰酸酯(ANIT)所致大鼠急性黄疸模型的退黄降酶作用。方法:将Wistar大鼠随机分为正常组、ANIT肝损伤模型组、GLP高(400 mg.kg-1)、中(200 mg.kg-1)、低(100 mg.kg-1)剂量组和阳性药组(茵栀黄颗粒)。各治疗组连续灌胃给药7 d后,ANIT诱发黄疸模型,48 h后摘眼球取血,分离血清,以血清肝功能指标、SOD的活力和MDA含量以及肝脏组织病理学的改变为观察指标。结果:与模型组比较,GLP高、中剂量组可不同程度的的降低ALT、AST、TBIL、ALP、γ-GT活性和MDA含量,升高SOD活性(P<0.01或P<0.05)。肝脏病理组织学检查表明,GLP能明显减轻肝细胞变性、坏死和肝小胆管增生。GLP高、中、低剂量组之间存在量效关系。结论:GLP具有降低实验性胆汁淤积大鼠血清胆红素、转氨酶和改善肝脏组织损伤的作用,其作用机制可能与抗氧化作用有关。

OBJECTIVE To observe the protective effect of Ganoderma lucidum polysaccharides （GLP） on liver injury in jaundice model of rat induced by α-naphthyl isothiocyanate （ANIT）. METHOD6 Rats were randomized into six groups： in group A no treatment was given, in group B was given on the seventh day, in group C, D, E, the high dosage （400 mg·kg^-1 ）, moderate dosage （200 mg·kg^-1） and tow dosage （100 mg·kg^-1 ） of GLP was given respectivety,in group F Yinzhihuang granule was given as positive control. Except group A and B, the other groups were given corresponding medicines for 7 days and then additionally administered ANIT to induced jaundice. After 48 h, the blood was taken from rats＇eyes. The serum was separated. The liver functional sign and the content of SOD and MDA in serum, and the change in pathology of liver tissue were observed. RECULTS Compared to group B, ALT, AST, TBIL, ALP, γ-GT, MDA of group C and D group dropped obviously, and SOD increased obviously （P〈0. 01 or P〈0. 05）. GLP could notably alleviate hepatocell pathalogical changes, necrosis and hepato-minicholange-hyperplasiA. CONCLUSION GLP has the action of reducing serum bilirubin and transarninase, improving liver tissue injury in rats of experimental cholestasis, the mechanisms of which is probably related to antioxidation.