摘要
本文采用体外培养人微血管内皮细胞(HMEC-1),不同浓度和时间的Julibroside J8干预,SRB法测定细胞存活率,同时结合DAPI荧光染色,TUNEL染色法检测细胞凋亡情况;Annexin-V/PI双标记行流式细胞仪测定定量观察细胞凋亡率。发现合欢皂甙Julibroside J8可显著抑制HMEC-1生长,抑制率可达75.6%,并呈时间-剂量效应关系。同时诱导细胞发生凋亡率,最高比例可达32.32%。
HMEC-1 cells were treated with different doses of Julibroside J8 and different time.The inhibition of cell growth was detected by using SRB assay.The TUNEL and DAPI fluorescent staining were measured in cells.Annexin-Ⅴ/PI double staining was used to measure the cell apoptosis rate by flow cytometry.The results showed that Julibroside J8 could inhibit the growth of HMEC-1 and induce the cell apoptosis.The biggest inhibition rate was up to 75.6% and the apoptosis rate was about 32.32%,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第3期432-435,共4页
Natural Product Research and Development
基金
2008教育部博士点新教师基金(200802951021)
