蝙蝠葛中的多巴胺能生物碱(英文)

Dopaminergic Alkaloids from Menispermum dauricum

目的:研究中药蝙蝠葛的化学成分。方法:应用硅胶和凝胶反复柱层析进行分离,分得成分用波谱分析和理化性质对照进行结构鉴定。结果:分离鉴定出8种成分,分别为meniscoside(1),dauricoside(2),dauriporphine(3),menisporphine(4),bianfugecine(5),2,3-dihydromenisporphine(6),syringaresinol(7)和syringaresinol4,4′-O-bis-glucoside(8)。结论:化合物1为新的苄基异喹啉生物碱葡萄糖苷,首次报道化合物1-6在对多巴胺D1和D2受体竞争性结合实验中,表现出对D1受体明显的选择性结合,其中1和2的IC50分别为9.2和2.0μmol?L-1。

AIM：To study the bioactive components of the rhizoma of Menispermum dauricum.METHODS：Repeated column chromatography was used for the separation of the compounds and various spectroscopic techniques were applied for structural determination.RESULTS：Eight compounds,meniscoside（1）,dauricoside（2）,dauriporphine（3）,menisporphine（4）,bianfugecine（5）,2,3-dihydromenisporphine（6）,syringaresinol（7）,and syringaresinol 4,4＇-O-bis-glucoside（8） were obtained and identified.The effects on D1 and D2 receptors labeled with [3H] were evaluated.CONCLUSION：Compound 1 was a new glucosidic benzylisoquinoline.Compounds 1-6 showed significant selective affinity to D1 receptor,and the IC50 of 1 and 2 were 9.2 and 2.0 μmol？L-1,respectively.