大胡蜂窝对酸性乙醇诱导的大鼠胃溃疡的保护作用和抗氧化活性(英文)

Protective and antioxidant properties of wasp (Vespamagnifica) honeycomb extract:a potential inhibitor againstacidified ethanol-induced gastric lesions

目的研究大胡蜂窝乙醇抽提物（WCE）对60%酸性乙醇诱导的大鼠胃溃疡的保护性及其作用机制。方法用WCE按照0、25、100和150mg/kg的剂量对Wistar大鼠连续灌胃8d,然后用60%的酸性乙醇诱导胃溃疡,比较不同处理组大鼠胃粘膜的病变程度、髓过氧物酶活性和总抗氧化能力,同时分析WCE体外抗二苯基三硝基肼自由基的能力。结果与0mg/kgWCE灌胃组相比,25、100和150mg/kgWCE灌胃组的胃溃疡程度下降了44.2%87.1%;胃粘膜髓过氧物酶活性下降了16.4%56.6%,胃粘膜的总抗氧化能力分别增加0.5、1.47和1.83倍。此外,WCE体外自由基清除能力显著高于二丁基羟基甲苯。结论通过降低胃粘膜自由基的破坏性和中性粒细胞的渗漏性,WCE能显著抑制60%酸性乙醇诱导的大鼠胃溃疡和溃疡形成过程中的氧化反应。

Objective To examine the protective effects of wasp（Vespa magnifica）honeycomb extract（WCE）against gastric lesions inrats induced by 60%acidified ethanol,and evaluate its capacity to suppress oxidative stress in the gastric tissue.Methods Wistarrats were subjected to intragastric administration of 60%acidified ethanol to induce gastric lesions following an 8-day oralpretreatment with WCE at 0,25,100 and 150 mg/kg or with saline.The levels of 1,1-diphenyl-2-picrylhydrazyl（DPPH）free radicalscavenging,myeloperoxidase（MPO）activity and total antioxidant capacity in the gastric tissues were determined.Results Oraladministration of 25,100 and 150 mg/kg WCE prior to 60%acidified ethanol administration significantly inhibited the formation ofgastric lesions（with a reduction by 44.2%-87.1%）,decreased the mucosal MPO activity（by 16.4%-56.6%）and increased the totalantioxidant capacity of the gastric tissue（by 0.5,1.47 and 1.83 folds,respectively）in a dose-dependent manner.At a highconcentration（above 1 mg/ml）,WCE also exhibited a stronger DPPH radical scavenging activity than butylated hydroxytoluene（BHT）.Conclusion The ethanol extract of wasp honeycombs can suppress the formation of acidified ethanol-induced gastric lesionsby reducing free radical oxidation and neutrophils infiltration in the gastric tissue in rats.