胶原类基因mRNA在口腔鳞癌组织中的表达

Investigation of mRNA expression of collagen genes in oral squamous cell carcinoma and paired normal tissue

目的研究胶原类基因COL1A1、COL1A2、COL4A1、COL4A2、COL5A2 mRNA在口腔鳞状细胞癌(OSCC)及配对正常口腔黏膜组织中的表达及意义。方法采用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔黏膜中胶原基因COL1A1、COL1A2、COL4A1、COL4A2、COL5A2 mRNA的表达;所得数据采用SAS6.12软件包进行t检验。结果 RT-PCR检测结果表明,COL1A1 mRNA在OSCC中的表达较其对照粘膜表达上调2.78倍(P<0.001)。COL1A2、COL4A1、COL4A2、COL5A2 mRNA在OSCC中的表达较其对照黏膜分别上调1.07、1.15、1.27、1.16倍(P>0.05)。结论在OSCC癌变过程中,COL1A1 mRNA过表达,可能是OSCC的一个靶基因;COL1A1在OSCC分类诊断中有应用价值。

Objective To investigate the mRNA expression of collagen genes in oral squamous cell carcinoma（OSCC） and paired normal oral mucosal tissue.Methods The differential mRNA expressions of collagen genes between 30 OSCC tissues and the paired normal oral mucosal tissues were detected by RT-PCR.Result The relative expression level of COL1A1 mRNA in the 30 cancerous tissues was up-regulated by 2.78 folds as compared with its expression in the paired normal samples,suggesting its significant overexpression in OSCC（P〈0.001）.The expression levels of COL1A2,COL4A1,COL4A2,and COL5A2 mRNA in the cancerous tissues were up-regulated by 1.07,1.15,1.27,and 1.16 folds compared to those in paired normal samples（P〉0.05）.Conclusion COL1A1 mRNA overexpression may play an important role in the carcinogenesis of OSCC and can be a potential molecular marker of OSCC.