食管鳞状细胞癌组织中肌动蛋白交联蛋白的表达和临床意义

Expression of fascin in human esophageal squamous cell carcinoma and its clinical significance

目的探讨食管鳞状细胞癌组织及正常食管粘膜组织中肌动蛋白交联蛋白(FASCIN)的蛋白表达情况,并分析其与食管鳞状细胞癌临床病理及患者5年生存率的关系。方法 241例食管癌患者均来自河南林州市。利用组织微阵列技术,采用免疫组化ABC法,分析241例食管鳞癌组织(观察组)和相应正常食管粘膜组织(对照组)中FASCIN蛋白的表达情况。结果食管鳞癌组织中FASCIN蛋白表达阳性率明显高于正常食管粘膜组织中的表达阳性率(68.9%vs15.5%,P<0.05);FASCIN蛋白高表达与淋巴结转移、TMN分期有关,而与年龄、性别、肿瘤细胞分化程度、和大体分型无关;FASCIN蛋白高表达的食管鳞癌患者的5年生存率明显低于FASCIN蛋白未高表达的食管鳞癌患者。结论 FASCIN蛋白的异常表达可能与食管鳞状细胞癌的发生、发展相关,检测FASCIN可能为食管癌预后判断提供依据。

Objective To investigate the role of fascin,an actin bundling protein,in the development and progression of human esophageal squamous cell carcinoma（ESCC）,and explore its association with the clinicopathologic characteristics and 5-year survival of the patients.Methods Using tissue array and immunohistochemistry,the expression of fascin was determined in 241 ESCC tissues and the corresponding normal esophageal mucosal tissues.Results ESCC tissues showed a significantly higher overexpression rate of fascin than the corresponding normal esophageal mucosal tissues（68.9% vs 15.5% ,P〈0.05）.The overexpression of fascin was correlated to lymph node metastasis and TMN stage,but not to the patients’age,gender,tumor differentiation and general classification.Survival analysis showed that abnormal expression of fascin was associated with the 5-year survival rate of patients with ESCC.Conclusions The abnormal expression of fascin may play an important role in the progression of ESCC,and detection of fascin expression may have important prognostic values.