平菇原生质体培养再生与子实体的形成

PROTOPLAST CULTURE REGENERATION AND FRUITING BODY FORMATION OF Pleurotus ostreatus

选用玻璃纸液体培养基培养的不同菌龄的菌丝体,分别注入用不同渗透稳定剂配制的1.5%溶壁酶进行酶解,释放出原生质体,再经浅层液体培养获得再生菌丝,可用于栽培,比对照形成子实体快,其产量略高于对照。

Fresh mycelium of different ages were selected from liquid medium cultures maintained in glassing and immersed in 1.5% Lywallzyme which were prepared with different osmoticas, round and transparent protoplasts with clear protoplast membrane began to be liberated in great quantity. After 2 day's culture in liquid medium, hypha regeneration was found. Two months later, normal fruiting bodies formed. These fruiting bodies had big pileus, normal color, and increased yield compared with control.