甘油致BALB／C小鼠急性肾损伤后肾脏观察

Observation of renal regeneration after acute renal injury in mice model induced by glucerin administration

目的观察甘油致小鼠急性肾脏损伤后肾脏修复的情况。方法肌肉注射甘油致小鼠发生急性肾脏损伤后出取肾脏组织，于损伤后的不同时间点进行肾脏组织形态学检测；同时，应用免疫组织化学技术检测核增殖抗原在肾脏组织的表达情况，分析肾脏损伤后的修复情况。结果肌肉注射甘油3d后小鼠开始出现急性肾脏损害的表现。主要表现为肾脏肿胀，肾脏指数增加，实验组与对照组相比t=2．262，P〈0．05，差异有统计学意义，肾小管腔内出现大量蛋白质样物质沉积，肾小管上皮细胞变性，坏死。于肌肉注射甘油3d后，免疫组织化学技术检测显示，小鼠损伤肾脏的肾小管出现大量核增殖抗原表达阳性的再生小管上皮细胞。结论肾小管损伤后，可以出现肾小管上皮细胞增殖进行损伤的修复。

Objective To observe the renal regeneration after acute renal injury established by glycerin administration in mice. Methods The mouse model was established by glycerin administration. Histological evaluation was performed via routine methods. Dynamic regeneration of renal was accessed by detecting proliferating cell nuclear antigen using immunohistochemical staining. Results At 3th day after glycerin administration, kidney tissues were collected and evaluated. Kidney swelled and renal index increased （compaired to control mice ,t = 2. 262 ,P 〈 0.05 ）. Renal tubules were filled with proteins mass. Renal tubele cells suffered with degeneration, necrosis and desquamation could be found. Renal regeneration in tubeles was detected by measuring proliferating ceil nuclear antigen. Conclusions Renal regeneration originates from residual tubular ceils after renal injury in mice model.