碱性磷酸酶mRNA在体内组织工程骨中的表达

Expression of alkaline phosphatase mRNA of tissue engineering bone in vivo

目的检测碱性磷酸酶(akp)mRNA在磷酸钙陶瓷(CPC)构建的体内组织工程骨中的表达,并将其与自然再生骨进行比较。方法将28粒CPC分别植入7只犬的竖直肌内,并拔除犬的1颗磨牙使之形成自然再生骨模型。术后3/7、1、2、4、8、12和24周,取出犬体内组织工程骨和自然再生骨,再以实时定量PCR检测其akp mRNA的表达。结果在试验周期内,akp mRNA在体内组织工程骨中表达达到峰值的时间较自然再生骨晚,且表达量少,但akp mRNA在这两种骨中均呈先上升后下降的现象。结论体内组织工程骨的钙化较自然再生骨晚,且量少,但骨形成过程中基因的表达趋势与自然再生骨相似。

Objective To detect the expression of alkaline phosphatase（akp） mRNA in in vivo tissue engineering bone constructed with calcium phosphate cement and do a comparison with that in naturally healing bone. Methods 28 porous calcium phosphate cement cylinders were implanted into the dorsal muscles of 7 dogs respectively and one molar was pulled out in each dog to create naturally healing bone model.The in vivo tissue engineering bone and the naturally healing bone were harvested at 3/7,1,2,4,8,12 and 24 weeks post-implantation.Then,akp mRNA was detected by real-time quantitative polymerase chain reaction（PCR）.Results In this experiment akp mRNA was expressed at lower levels with delayed peak in in vivo tissue engineering bone than that in naturally healing bone.However,the expression of akp mRNA shared a similar pattern of initially upregulated and finally down-regulated in the two kinds of bone.Conclusion The in vivo tissue engineering bone is calcified later and fewer than natrually healing bone.However,the gene expression pattern is similar to that of naturally healing bone in bone formation.