摘要
目的:探讨异莲心碱对D-半乳糖致衰老小鼠抗氧化的作用。方法:选取昆明种成年小鼠50只,随机将其分为空白组、模型组、异莲心碱低剂量组(10 mg/kg)、高剂量组(20 mg/kg)及维生素C(Vitamin C)对照组,测定小鼠体重和肝脏指数,以小鼠血清和肝脏组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及MDA含量为指标,考察异莲心碱在体内的抗氧化性能。结果:与空白组比较,模型组小鼠体重和肝脏指数明显下降,为32.87 g和3.87 mg/g;血清和肝脏组织SOD和GSH-Px活性明显降低,其值分别为247 U/ml、408 U/ml和358、319 U/mgpro;MDA含量为20.62 nmol/ml和13.01 nmol/mgpro,明显增加(P<0.01);与模型组比较,异莲心碱20 mg/kg可明显拮抗小鼠体重和肝脏指数的降低、增强血清和肝脏组织SOD和GSH-Px活性、减少MDA含量(P<0.01),其值分别为36.29 g,4.53 mg/g,305、459 U/ml,438、355 U/mgpro和16.47 nmol/ml。异莲心碱低剂量组小鼠肝脏组织GSH-Px活性为333 U/mgpro,与模型组比较差异无统计学意义。结论:异莲心碱能显著提高D-半乳糖致衰老小鼠的抗氧化能力。
Objective:To study the antioxidative effect of isoliensinine in D-Galactose-induced aged mice.Methods:The fifty mouses were randomly divided into five groups involved in black group,model group,low-dose isoliensinine group(10 mg/kg),high-dose isoliensinine group(20 mg/kg) and the group treated with Vitamin C.The body weight and the liver index were determined and the activities of super-oxide dismutase(SOD),glutathione peroxidase(GSH-Px) and the contents of malonic dialdehyde(MDA) in serum and liver tissue were assayed respectively.Results:Compared with black group,for aging model mice,the body weight and the liver index declined,the data were 32.87 g and 3.87 mg/g,the activities of SOD and GSH-Px in surum and liver tissue also declined,the data were 247,408 U/ml and 358,319 U/mgpro,and the contents of MDA raised,the data were 20.62 nmol/ml and 13.01 nmol/mgpro(P0.01).In comparison with the model group,the loss of body weight and the liver index were markedly counteracted,the activities of SOD and GSH-Px in surum and liver tissue of isoliensinine group(20 mg/kg) were all increased and the contents of MDA got high reduction(P0.01),the data were 36.29 g,4.53 mg/g,305,459 U/ml,438,355 U/mgpro and 16.47 nmol/ml respectively.The activities of GSH-Px in liver tissue was 333 U/mgpro and had no marked effect compared with model group.Conclusion:Isoliensinine can significantly increase the antioxidative effect of D-Galactose-induced aged mice.
出处
《中国医药导报》
CAS
2011年第20期5-6,9,共3页
China Medical Herald