孕妇产前免疫性IgG抗体效价检测意义探讨

Effect of IgG-type antibody on pregnant woman

目的:探讨夫妇ABO/RhD血型不合妊娠期血型抗体效价与妊娠次数及年龄的关系。方法:采用试管凝集法常规检测夫妇ABO及RhD血型,对夫妇ABO及RhD血型不合的孕妇进行血清采用凝聚胺法微柱凝胶法不规则抗体筛选、鉴定和IgG-A或IgG-B及抗-D效价测定;分析IgG-A或IgG-B效价与妊娠次数及年龄段的统计学关系。结果:380例ABO血型不合夫妇的孕妇血清中检出IgG-A或IgG-B效价≥1∶64者204例(53.6%),在8例RhD阴性孕妇中检出抗-D 5例(62.5%)。多次妊娠孕妇的IgG-A或IgG-B效价≥1∶64明显多于1次妊娠者(P<0.01);31～40岁的高龄孕妇的IgG-A或IgG-B效价≥1∶64高于21～30岁的适龄孕妇(P<0.01)。结论:产前对夫妇进行ABO/RhD血型、IgG-A或IgG-B、抗-D抗体效价检测,对妊娠次数较多、高龄孕妇在孕早期即应进行定期抗体效价检测,以便及时预防、诊断、治疗,减少母婴血型不合新生儿溶血病的发生。

Objective：To investigate the relationship of antibody titer,pregnancies number,age on couples with ABO/RhD blood type incompatibility.Methods：ABO/RhD blood type were detected by tube agglutination and irregular antibody screen or idenfication were tested by ploybere or microcolumn gel,The relationship between IgG-A or IgG-B aitibody titer and pregnancies number and age were anlyzed.Results：IgG-A or IgG-B aitibody titer in 204（53.6%） samples were more than 1：64 from total 380 samples with ABO blood type incompatibility.5（62.5%） samples were detected anti-D in 8 samples whose RhD blood type are negtive,IgG-A or IgG-B aitibody titer ≥1∶64 in more pregnancy were more than only once pregnancy（P0.01）,and eld pregnency with 31 to 40 years old were high than 21 to 30 years old pregnancy（P0.01）.Conclusion：Detecting the ABO/RhD type blood,irregular antibody screen or IgG-A or IgG-B aitibody titer regularly on more pregnancy or eld pregnancy have an importang effect on prophylaxia,diagnosis,treatment in order to reduce hemolytic disease of newborn（HDN）.