茵陈蒿汤方剂配伍对急性胰腺炎小鼠模型作用的研究

Research of Herbae Artemisiae Capillariae Decoction and Its Components' Effect on Mice Model with Acute Pancreatitis

目的:通过茵陈蒿汤(HACD)拆方配伍对急性胰腺炎(AP)小鼠胰腺组织炎症病理和IL-6表达的影响,探讨HACD的方剂配伍规律对AP小鼠模型的作用。方法:选择健康昆明小鼠90只,分9组:正常对照组、AP模型组、HACD全方组、拆方配伍组(6组),每组各10只。选择间隔1h两次腹腔注射L-精氨酸(L-Arg)(200mg/100g体重)的方法制模,在注射L-Arg后24h,对不同组别进行胰腺组织炎症病理评分分析,并用ELISA法检测血清IL-6的浓度,应用RT-PCR法检测胰腺组织的IL-6mRNA。结果:腹腔内注射L-Arg后24h,全方组小鼠胰腺病理评分分值最低,其血清IL-6浓度与胰腺组织IL-6mRNA表达量最小,分别与拆方后的6组以及模型组比较,差异均有显著性意义(P<0.01);有大黄的组别(大黄+栀子组、大黄+茵陈组、大黄组)胰腺病理评分、血清IL-6浓度与胰腺组织IL-6mRNA表达亦显著降低,对比无大黄组(栀子+茵陈组、茵陈组、栀子组)和模型组,差异均有显著性意义(P<0.01)。结论:全方组对减轻AP的炎症反应最为显著,在HACD治疗AP中,大黄可能起君药作用,茵陈、栀子对大黄作用可能有协同或相加效应。

Objective：To investigate the effect of Herbae Artemisiae Capillariae Decoction（HACD） and its components on mice model with acute pancreatitis by the function of HACD and its components on the pancreatic inflammatory pathologic reaction and the IL-6 expression in serum and pancreas in acute pancreatitis mice.Methods：90 healthy Kunming mice were divided randomly into 9 groups：normal contrast group,model group,HACD group,the six components-treated groups,there were 10 mice in every group（n=10）.Acute pancreatitis was induced by intraperitoneal injection of L-arginine（L-Arg）（200 mg/100g body weight） twice at an interval of one hour.The pancreatic inflammatory pathologic reaction was scored and analyzed,the IL-6 serum concentration was measured by ELISA and the IL-6 mRNA expression of pancreatic tissues was detected by RT-PCR at 24h after L-Arg injection in different groups.Results：At 24h after L-Arg injection,the histological scoring on AP pancreatic tissue was lowest and the IL-6 expression in serum and pancreas was least in HACD-treated group.The difference existed notable significance compared with the components-treated groups and model group（P0.01）.In including-Rhubarb groups（rhubarb group,rhubarb and gardenia group,rhubarb and herba group）the histological scoring and the IL-6 expression was reduced significantly more than those of no-Rhubarb groups（gardenia group,herba group,gardenia and herba group）（P0.01）.Conclusion：HACD may markedly relieve the inflammation reaction in AP mice,and the rhubarb may play a primary role in the treatment of AP,meanwhile the herba and gardenia cooperate with it.