摘要
目的对中国恒河猴主要组织相容性复合体(MHC)I型部分基因进行携带情况调查与分析。方法采用序列特异性引物(PCR-SSP)分型方法对华南灵长类动物研究中心繁殖的30只谱系清晰的中国恒河猴(Macacamulatta)的32个MHC I型分子位点进行检测。结果采用的32对引物中,中国恒河猴可检出携带23个MHC I等位基因,但基因携带频率存在很大的差异,由3.57%至82.14%不等。结合遗传谱系分析,判断A1*21和A2*05之间以及B*04和B*30之间可能就是连锁的。结论中国恒河猴携带能控制病毒复制的MHC I型基因位点的频率较高,其基因携带频率与已发表的印度恒河猴携带频率存在明显差异。本研究为促进中国恒河猴在AIDS研究中的应用,以及为建立携带特定MHC I基因实验猴小种群提供了依据。
Objective To genotype and analyze the MHC class I alleles in Chinese rhesus macaques（Macaca mulatta）.Methods PCR amplification with sequence-specific primers（PCR-SSP） for 32 loci was carried out for genotyping of MHC class I alleles in Chinese rhesus monkeys derived from Guangxi group（n=30）.Results In Chinese rhesus macaques,twenty-three MHC class I loci were detected with frequency varying from 3.57% to 82.14%.Based on their genetic lineages,mamu-A1＊21 may be linked with A2＊05 and mamu-B＊04 may be linked with B＊30.Conclusion High frequency of SIV-limiting MHC class I loci occurs in Chinese rhesus macaques,which is different from that of Indian monkeys.Our results may facilitate the selection,breeding and utilization of Chinese rhesus monkeys in AIDS pathology and vaccine studies.
出处
《实验动物科学》
2011年第3期42-46,50,共6页
Laboratory Animal Science
基金
广州市重大科技专项(穂科条[2008]4-01)
广州市重大科技专项(No:2009A1-E051)
广东省科技计划项目(No:2010B060500023)
