复方蜥蜴散不同微粒组合剂治疗大鼠慢性萎缩性胃炎的实验研究

Experimental Study of the Different Combination of Compound Lizards Powder on Chronic Atrophic Gastritis in Rats

目的:观察复方蜥蜴散不同微粒组合剂对慢性萎缩性胃炎(Chronic atrophic gastritis,CAG)模型大鼠血清PGE2及NOS水平的影响,探讨其胃黏膜保护作用机制。方法:将90只SD雄鼠随机分为正常组、模型组、复方蜥蜴散80目组、复方蜥蜴散100目组、复方蜥蜴散不同微粒组合剂(80目+100目)组及维酶素组。除正常组外,其余各组采取2%水杨酸、55℃热盐水经口灌胃,20mmoL/L脱氧胆酸钠自由饮用,配合饥饱失常造成CAG模型。治疗后4周,检测大鼠血清PGE2及NOS的水平。结果:复方蜥蜴散各不同微粒剂型治疗组大鼠PGE2水平明显高于模型组(P<0.01),NOS水平则低于模型组(P<0.01);复方蜥蜴散不同微粒组合剂组大鼠PGE2水平高于复方蜥蜴散80目组和100目组(P<0.01),NOS水平则低于复方蜥蜴散80目组和100目组(P<0.01);各治疗组胃黏膜病理表现明显好转。结论:复方蜥蜴散各不同微粒剂型均可改善CAG模型大鼠胃黏膜的病理情况,升高PGE2水平,降低NOS水平,发挥保护胃黏膜的作用;其中以复方蜥蜴散不同微粒组合剂疗效最佳。

Objective：To observe the effect of the Different Combination of Compound Lizards Powder（DCCLP） on serum PGE2 and NOS of chronic atrophic gastritis（CAG） model rats,and explore the mechanism of gastric mucosal protection.Methods：90 SD male rats were randomly divided into normal group,model group,compound powder lizards 80 mesh group,compound powder lizards 100 mesh group,the DCCLP（80 mesh＋100 mesh） group and the Vitamycin group.In addition to the normal group,other groups take 2% salicylic acid,55 ℃ hot saline water by gavage,20mmoL/L sodium deoxycholate freely drinking,with irregular diet caused CAG model.After 4 weeks treatment,serum PGE2 and NOS were detected.Results：The PGE2 levels of the DCCLP group was significantly higher than the model group（P0.01）,NOS was lower than the model group（P0.01）;PGE2 levels of the DCCLP group higher than 80 mesh and 100 mesh group（P0.01）,NOS was lower than the 80 mesh and 100 mesh group（P0.01）,the mucosal pathology has significant reversing.Conclusion：The DCCLP may reverse the gastric mucosa pathology in CAG model rats,increase PGE2 levels and reduce NOS levels to play the role of protecting gastric mucosa;which the DCCLP has the best clinical therapeutic effect.