西花蓟马检测鉴定技术研究进展

Advances in identification techniques of the western flower thrips,Frankliniella occidentalis(Pargande)

西花蓟马是一种世界性的入侵害虫,2003年首次在北京发现,目前仅在我国局部地区发生。其危害严重,潜在适生区广,且具有进一步扩散蔓延的趋势。快速准确的检测鉴定技术是防止其进一步扩散蔓延,保障我国农业生产健康发展的必要前提。综合相关报道,用于西花蓟马的检测鉴定技术包括传统的形态学特征鉴定法、由此衍生的计算机软件识别法,以及分子检测技术,如RAPD-PCR法、DNA序列分析法、PCR-RFLP法、SCAR分子标记技术、实时荧光定量PCR检测技术以及蛋白质分析技术等。其中,基因芯片和DNA条形编码技术作为新兴的物种鉴定手段,在西花蓟马等入侵物种的检测鉴定中具有广阔的应用前景。

The western flower thrips, Frankliniella occidentalis （Pargande）, a globally invasive species, was first reported in Beijing in 2003 ; it currently occurs only in a few areas in China. Considering its devastating effects, a rapid and simple method for the identification of this species would facilitate decision-making regarding plant stock movements, help in preventing its introduction and spread to non-infested areas. There are several methods in use to identify F. occ/denta//s, including morphological identification, computer-assisted identification by using compact disc read-only memory （CD-ROM） or artificial neural networks （ANN）. Molecu- lar identification methods based on RAPD analysis, COI gene sequencing, RFLP associated with COI, cyt b, ITS-1 and ITS-2 genes as well as protein-based analysis including Mab-ELISA and SDS-PAGE have been developed. Recently, species-specific primers based on genomic DNA （SCAR marker） or mtDNA COI, as well as real-time quantitative PCR-assisted by SCAR or COI markers have become available. New identification methods derived from gene chip and DNA barcoding are in development. Of the available methods, techniques utilising gene chips and DNA barcoding are expected to give more stable and reliable results and will be the most practical methods in identification of the western flower thrips and other alien species.