龙牙百合花氨基酸含量的柱前衍生OPA-HPLC法测定

Determination of Amino Acid in Flower of Lilium brownii var.viridulum by Reversed-phase High-Performance Liquid Chromatography with Pre-column Derivation

[目的]测定龙牙百合花中的氨基酸含量。[方法]以邻苯二甲醛(OPA)与9-芴基羰酰氯(FMOC-Cl)作衍生剂,利用VWD检测器可变波长检测程序,采用柱前衍生OPA-HPLC法测定邵阳龙牙百合花中的17种氨基酸含量。[结果]邵阳龙牙百合花中含有苏氨酸、丝氨酸、脯氨酸、蛋氨酸、谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸、天门冬氨酸、丙氨酸、组氨酸、半胱氨酸、异亮氨酸等17种氨基酸。其中必需氨基酸的含量为49.7 mg/g,占氨基酸总量的31.20%;儿童必需氨基酸的含量为30.6 mg/g,占氨基酸总量的19.20%;含量最高的是谷氨酸(28.7 mg/g,占氨基酸总量的18.03%);其次是精氨酸(27.5 mg/g,占氨基酸总量的17.30%)。[结论]该方法操作简便,灵敏度高,结果准确,可靠。龙牙百合花中氨基酸的种类齐全,含量丰富,具有很高的开发利用价值。

[ Objective ] To determine the contents of amino acids in flower of Lilium brownii var. viridulum. [ Methdd ] Contents of 17 amino acids in flower of Lilium brownii var. viridulum were determined using OPA-HPLC method, in which o-phthalaldehyde （OPA） and 9-fluore- nylmetloy chorofomnate （FMOC） were selected as pre-column derivative reagent and VWD detector was used. （Result] The Lilium brownii var. viridulum from Shaoyang was rich in Asp, Glu, Ser, His, Gly, Thr, Arg, Ala, Tyr, Cys, Val, Met, Phe, Iso, Leu, Lys and Pro. The contents of essential amino acids for adult and for children were 49.7 mg/g and 30.6 rag/g, respectively, which were 31.20% and 19.20% of total contents of amino acids individually. Content of Glu and Arg in samples ranked the first and second,＇ respectively, which were 28.7 and 27.5 mg/g respectively, taking up 18.03% and 17.30% of total amino acids. [ Conclusion] The method is simple in operation, high in accuracy and accurate in determining re- sult. Flower of Lilium brownii var. viridulum are rich amino acids, which is of high developing value.