转基因败育毛白杨雄株试管苗气孔行为观察

Observation of the Stomatal Behavior of Test-tube Plantlet of Transgenic Male Abortive Populus tomentosa Carr

[目的]探寻试管苗叶片气孔行为异常的原因。[方法]利用指甲油涂抹撕取法将转基因败育毛白杨雄株试管苗与10年生植株叶片下表皮制成切片,并对其气孔密度、气孔大小、气孔开度日变化、气孔开张率日变化进行观察比较。[结果]试管苗气孔密度小于10年生植株;试管苗气孔明显比10年生植株气孔大;试管苗气孔开度日变化呈明显波峰状,2个高峰分别出现在9:00和15:00,10年生植株曲线平稳,无明显波动,且试管苗气孔开度大于10年生植株;试管苗气孔开张率在一昼夜均为100%,10年生植株气孔开张率在一昼夜呈双曲线变化。[结论]转基因败育毛白杨雄株试管苗与10年生植株气孔行为存在差异。

[ Objective ] To study the causes of abnomml stomata1 behavior of test-tube plantlet. [ Method ] The change of stomatal density,stomatal size ,the daily variation of stomatal aperture and stomatal open percentage of the leaves＇ lower epidermis of test-tube plantlets and 10-year-old plants of transgenic male abortive Populus tomentosa Carr were observed and compared in this paper by using nail polish method. [ Result ] The stomatal density of test-tube plantlets was less than that of the 10-year-old plant ; test-tube plantlets＇ stomata was obviously larger than that of the 10-year-old plant; the stomatal aperture of test-tube plantlets showed wave crest, and the two peaks appeared at 9：00 and 15：00,10-year-old plant had steady curve without evident fluctuation,and the stomatal aperture in test-tabe plantlets was greater than that in the l O-year-old plant; stoma- tal open percentage reached 100% in test-tube plantlets during a day,a bimodal curve change was observed in stomatal open percentage of the lO- year-old plant. [Conclusion] Stomatal behavior were different between the test-tube plantlets and the lO-year-old plant of transgenic male abortive Populus tomentosa Cart.