抗松针褐斑病湿地松子代组培苗瓶内菌根化研究

Study on the Mycorrhizal Formation in the Offspring Tissue Culture Bottle of Pinus elliottii with the Brown Spot Needle Blight Resistance

[目的]研究抗松针褐斑病湿地松子代组培苗瓶内菌根化。[方法]对湿地松再生植株离体条件下菌根的形成进行了研究,并对再生植株的生长状况进行了观察。[结果]菌根真菌培养基质和接种量对离体条件下菌根的形成有较大影响。湿地松丛生芽诱导出根原基后,以转接到珍珠岩为培养基质接种彩色豆马勃为最佳,有利于菌根的形成;在珍珠岩接种彩色豆马勃为2个菌块时,菌根化率高达84.4%,形成的二叉分枝状短根最多,平均每条主根上形成12.49条;菌根的形成提高了再生植株的驯化移栽成活率,菌根化再生植株在植物生长室长势良好,根系发达。[结论]为提高抗松针褐斑病湿地松组培再生植株的成活率提供了依据。

[Objective J The research aimed to study the mycorrhizal lormatlon m the oitsprmg tissue culture bottle ot r＇mus etttotttt with the brown spot needle blight resistance. [ Method] The mycorrhizal formation under the vitro condition of Pinus elliottii regenerated plant was stud- ied, and the growth condition of regenerated plant was observed. [Result] The mycorrhiza fungi culture substrate and the inoculation amount had the big influence on the mycorrhizal formation under the vitro condition. After the primordial rooting was induced from Pinus elliottii buds, it was best to be transplanted into the perlite medium and inoculate Pisolithus tinctorius, which was favorable for the mycorrhizal formation. When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium, the mycorrhizal formation rate reached 84.4%. The dichot- omous branching short root was the most, and 12.49 roots were formed on every main root. The mycorrhizal formation improved the survival rate of regenerated plant domestication transplant. The mycoIThizal formation regenerated plant grew well in the phytotron, and the root system was developed. [ Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with the brown snot needle blight resistance.