绿玉树茎段组织培养再生体系的建立

Establishment of Tissue Culture Regeneration Systems of Stem Segments of Euphorbia tirucalli

[目的]研究绿玉树茎段组织培养再生苗的条件,确定各培养阶段的最佳培养条件,为绿玉树组培苗工厂化生产和相关研究提供参考。[方法]以绿玉树茎段作为外植体试验材料,研究了不同培养基对萌芽率、增殖倍数、生根率的影响。[结果]萌芽培养最佳的诱导培养基为1/2MS+0.02mg/LNAA+1.00mg/L6-BA,分化率为89.7%;继代培养最佳培养基为1/2MS+0.02mg/LNAA+0.60mg/L6-BA+3.00mg/LAD,增殖倍数为5.70;生根培养最佳培养基为1/2MS+0.40mg/LNAA+0.40mg/LIBA,生根率达100%,移栽成活率达80%。[结论]初步确定了绿玉树茎段组织培养的生长条件。

[ Objective ] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tiru- calli and determine the optimum culture condition of each culture stage, so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [ Method ] Taking the stem segments of E. tirucalli as explants, the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [ Result] The optimum induction medium of germination culture was 1/2MS ＋ 0.02 mg/L NAA ＋ 1.00 mg/L 6-BA ,with differentiation rate of 89.7% ;the best subculture medium was I/2MS ＋0.02 mg/L NAA ＋0.60 mg/L 6-BA ＋ 3.00 mg/L AD, with multiplication coefficient of 5.70 ;the optimum rooting culture medium was 1/2MS ＋ O. 40 mg/L NAA ＋ 0. 40 mg/L IBA, with rooting rate of 100% and transplanting survival rate of 80%. [ Conclusion ] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.