板栗分子标记辅助育种的配套育苗技术研究

The Supporting Nursing Technology about the Castanea mollissima Blume Marker Assisted Selection

[目的]研究板栗分子标记辅助育种的配套育苗技术,以期节约分子标记辅助育种苗木培育成本、缩短育种年限。[方法]按照板栗的横径和纵径,采取1/2、1/3、1/4等切取比例以及横切、竖切、斜切、双边竖切等切取方法,共计10个处理。测定各处理的发芽率、成活率及苗木形态指标,分析各处理与对照之间的显著性差异。[结果]1/3斜切为最佳切取方式,不仅可以保证高发芽率、成活率,且经其处理后的幼苗生长状况与对照的板栗幼苗无明显差异,苗木质量较高。[结论]该套育苗技术,不仅可以节约育苗成本,缩短育种年限,还可促进板栗提前发芽,对生产有一定的指导意义。

[ Objective ] The paper aimed at studying the supporting technology of Castanea moUissima Blume molecular marker-assisted selec- tion to decrease the cost and reduce time for chestnut nursing. [ Method] To find the best cutting method, 10 kinds of treatments were de- signed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2, 1/3 ~ 1/4 and different directions about cross cutting, vertical cutting, bevel cutting and two side-cutting. The germination rate, seedling survival rate and morphologi- cal indicators of the treatments were measured ; significant difference among them was also analyzed. [ Result ] 1/3 bevel cutting was the best, which not only could give high germination rate and survival rate, but also could guarantee high quality of nursery stock, which did not grow so differently with the untreated seedlings. [ Conclusion] This way can not only decrease much cost and time for seedling breeding, but also promote the germination of chestnut in advance, which could do much good to the production.