多环芳烃厌氧降解菌的筛选与鉴定

Screening and Identification of Two Anaerobic PAHs-biodegrading Strains

[目的]筛选并鉴定多环芳烃厌氧降解菌。[方法]通过富集,在厌氧条件下从受焦油长期污染的土壤中筛选出多环芳烃的高效厌氧降解菌,并对其进行了生理生化试验和16SrDNA鉴定。[结果]从受焦油长期污染的土壤中分离出2株多环芳烃降解菌W2和Y3,经综合表征和16SrDNA序列分析,初步鉴定菌株W2为鞘氨醇单胞菌属(Sphingomonas sp.),菌株Y3为芽孢杆菌属(Bacillus sp.)。[结论]为多环芳烃的生物降解研究提供了理论依据。

[ Objective ] The aim was to screen and identify anaerobic PAHs-biodegrading strains. [ Method J High-efficient anaerobic PAHs- biodegrading strains were screened out from the soil which was polluted by tar oil for a long time, and then carried out a physiological biochem- ical test and 16S rDNA identification. [ Result] Two anaerobic PAHs-biodegrading strains like W2 and Y3 were isolated from the soil which was polluted by tar oil for a long time. According to comprehensive characterization and 16S rDNA sequence analysis, W2 was preliminarily i- dentified as Sphingomonaz sp. , and Y3 was preliminarily identified as Bacillus sp., [ Conclusion ] The researeh provides theoretical basis for studies on PAHs biodegradation.