摘要
目的:优选超声波辅助提取连翘中总黄酮的工艺参数。方法:采用单因素和正交试验进行超声波辅助提取连翘总黄酮,以紫外分光光度法测定连翘总黄酮的吸光度,标准曲线法计算含量。结果:最佳工艺条件为65%乙醇60℃超声提取10 min,连翘总黄酮的平均质量分数为2.58%。结论:优化工艺简单,稳定,高效,可行。
Objective：To establish optimal process of extracting total flavonoids from Forsythiae Fructus by single factor test and orthogonal test.Method： The absorption of total flavonoids was determined by uv-spectrophotometer and the standard curve was used to calculate the content.Result： The optimum conditions for extracting the total flavonoids assisted by ultrasonic wave were as follow： 65% ethanol,ultrasonic time 15 min and extraction temperature 60 ℃.The mass fraction of total flavonoids extracted was up to 2.58%.Conclusion： The optimum extraction process was simple,stable,efficient and feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期30-32,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省科技创新杰出青年基金项目(104100510016)
新乡医学院省级重点学科开发课题(ZD200968)
关键词
超声提取
连翘
总黄酮
正交试验
ultrasonic wave extraction
Forsythiae Fructus
total flavonoids
orthogonal test
