牛蒡甘草汤的提取工艺优选

Optimization of Extraction Conditions of Niubang Gancao Tang by Orthogonal Design

目的:优选牛蒡甘草汤的最佳提取工艺条件。方法:以牛蒡苷含量和干膏得率的综合评分为指标,采用L9(34)正交设计法,对影响其提取率的重要因素(A)加水量、(B)煎煮时间、(C)煎煮次数进行优选。采用HPLC测定牛蒡苷的含量,Hypersil-Keystone C18色谱柱,流动相甲醇-水(43∶57),检测波长280 nm。结果:优选的最佳水提工艺条件为8倍量水,煎煮3次,每次2 h。验证试验显示牛蒡苷质量分数为20.69%,干膏得率为24.37%。结论:优选出的牛蒡甘草汤水提取工艺稳定可行。

Objective：To optimize the water extraction conditions of Niubang Gancao Tang.Method： The L9（3）4 orthogonal method was employed to study the best extracted technics through analyzing the content of arctiin by HPLC and the weight of ointment.The determination was carried out on a Hypersil-Keystone C18 column with the mobile phase of methanol and water（43∶57）,detection wavelength of 280 nm.Result： The best technics of extraction process is 10 times amount of water,decocted 3 times,2 hours each time.Validatvon test showed that 20.69% arctiin content,dry extract yield was 24.37%.Conclusion： Optimum conditions of water extraction of Niubang Gancao Tang are obtained and the optimized extraction technology is stable and efficient.