摘要
目的:建立广西主产山豆根药材的HPLC指纹图谱。方法:以氧化苦参碱为参照物,采用C18色谱柱,以甲醇-水(0.2%磷酸+0.32%三乙胺)为流动相,梯度洗脱,流速1.0 mL.min-1,柱温25℃,检测波长24 min后由220 nm转换到280nm。结果:其重复性、精密度均符合《中药注射剂指纹图谱研究的技术要求(暂行)》中的有关规定。结论:本实验结果为广豆根药材的质量控制提供了参考。
Objective：RP-HPLC fingerprint of Sophora tonkinensis grown in Guangxi Zhuang autonomous region was established.Method： A gradient separated method was applied on C18 column at 25 ℃ with methanol-water as mobile phase.Reference material was oxymatrine.Flow rate was 1.0 mL·min-1;detection wavelength was set up at 220 nm and changed to 280 nm after 24 min.Result： The results showed that this method had a good repeatability and precision.Conclusion： This result can provide scientific data for analyzing the quality control of Sophora tonkinensis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第14期66-68,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技攻关项目(桂科能0815005-1-9)
