高效毛细管电泳测定小果博落回中血根碱和白屈菜红碱

Content Determination of Sanguinarine and Chelerythrine In Macleaya microcarpa by HPCE

目的:建立测定小果博落回中血根碱和白屈菜红碱的含量的新方法。方法:采用高效毛细管电泳法,盐酸小檗碱为内标,运行缓冲液为25 mmol.L-1磷酸氢二钠与25 mmol.L-1磷酸二氢钠的混合体系(磷酸调pH为7.0)-甲醇(2.5∶1);运行电压18 kV;柱温25℃;检测波长200 nm。结果:血根碱和白屈菜红碱的线性范围分别为0.0124～0.0828mg.mL-1(r=0.999 8);白屈菜红碱的线性范围0.018 8～0.125 6 mg.mL-1(r=0.999 6),平均加样回收率(n=6)分别为98.7%(RSD1.01%)、98.4%(RSD 1.27%)。结论:高效毛细管电泳操作简便,分析快速,结果准确、重现性好,适用于小果博落回中血根碱和白屈菜红碱的含量的测定。

Objective：To establish a new method for determination of sanguinarine and chelerythrine in Macleaya microcarpa（Maxim）.Method： Sanguinarine and chelerythrine in Macleaya microcarpa were determined by using high performance capillary electrophoresis（HPCE） with berberine as internal standard.The background electrolyte system selected was 25 mmol·L-1 disodium hydrogen phosphate and 25 mmol·L-1 sodium dihydrogen phosphate buffer at pH=7-methanol（2.5∶1）;applied voltage,18 kV;temperature,25 ℃.Diode array detector was set at 200 nm.Result： The linear range of sanguinarine and chelerythrine was 0.012 4-0.082 8 g·L-1（r=0.999 8） and 0.018 8-0.125 6 g·L-1（r=0.999 6）,The average recovery was 98.7%（RSD 1.01%） and 98.4%（RSD 1.27%）.Conclusion： The method is simple,rapid and reproducible,and could be used for content determination of sanguinarine and chelerythrine In Macleaya microcarpa.