反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量

Determination of Rutin and Quercetin in Tea of Lycium barbarum Leaves by RP-HPLC

目的:采用反相高效液相法测定新疆枸杞叶茶中的芦丁和槲皮素的含量。方法:Kromasil C18色谱柱(4.6 mm×250mm,5μm),流动相甲醇-0.04%磷酸水溶液(用三乙胺调pH 3.0)(45∶55),检测波长360 nm,流速1.0 mL.min-1。结果:芦丁和槲皮素的线性范围分别在0.23～1.84μg和0.03～0.24μg,回收率分别为98.19%,97.98%。RSD分别为1.49%,2.25%。结论:本方法简便、可靠、重现性较好,为新疆枸杞叶茶的质量控制提供了快速、准确的测定方法。

Objective：To determine active constituents quercetin and rutin in tea of Lycium barbarum leaves by RP-HPLC.Method： Rutin and quercertin were separated on an Kromasil C18 column（4.6 mm×250 mm,5 μm） with methanol-0.04% phosphoric acid（pH was adjusted to 3 with diethylamide）（45∶55） as mobile phases.The detecting wavelength was at 360 nm.The flow rate was 1.0 mL·min-1.Result： The linear ranges of rutin and quercertin were within 0.23-1.84 μg and 0.03-0.24 μg respectively.The recoveries were 98.19% and 97.98%,RSDs were 1.49% and 2.25% respectively.Conclusion： The method is simple,accurate,and reliable,which provides the basis of a better development for the tea of L.barbarum in Xinjiang.