不同剂量化学纯三聚氰胺诱发SD大鼠尿结石的比较

Different Doses of Chemical Pure Melamine-induced SD Rats Comparison of Urinary Stone

目的:探讨建立不合并肾损害的尿结石模型。方法:100只雄性SD大鼠,随机分5组,空白对照组、化学纯三聚氰胺0.4～0.7 g.kg-1.d-1组,连续给药20,30 d,观察比较结石形成率、肾功能损害情况。结果:0.4,0.5 g.kg-1.d-1化学纯三聚氰胺给药20,30 d,膀胱结石率最高,20 d分别为60%,50%,30 d分别为50%,60%,两组及与0.6,0.7 g.kg-1.d-1组、空白对照组比较有显著性差异(P<0.05);膀胱结石形成率与剂量、时间不成线形关系;各组比较仅0.4 g.kg-1.d-1组肾和膀胱指数、肌酐、尿素氮、尿酸与空白对照组比较,无显著性差异。结论:化学纯三聚氰胺0.4 g.kg-1.d-1给药20 d对肾脏几乎不造成损害,SD大鼠膀胱结石率达60%,可建立不合并肾损害的尿结石模型。

Objective：To explore the establishment of non-renal injury model of urinary stone.Method： One hurdred SPF male SD rats were divided randomly into five groups.We administrated the animals chemical pure melamine 0.4-0.7 g·kg-1·d-1continuously 20 d and 30 d,then observed and compared the rate of stone formation and renal damage.Result： Administration of chemical pure melamine 0.4,0.5 g·kg-1·d-1,20,30 d,gained the highest rate of bladder stones formation,respectively,60% and 50% in 20 d,50% and 60% in 30 d.The difference of rate of bladder stones formation between group 0.4 g·kg-1·d-1 and 0.5 g·kg-1·d-1,and between group 0.4,0.5 and 0.6,0.7 g·kg-1·d-1,blank control group were significant（P0.05）.Rate of bladder stone formation was not correrated with the increase in dose and time.The group 0.4 g·kg-1·d-1is the only group that has no significant difference in the compare of renal and bladder index,creatinine,urea nitrogen,uric acid with the control group.Conclusion： Administration of chemical pure melamine 0.4 g·kg-1·d-1 did not cause kidneys injury and was able to induce high rate of SD rat bladder stone formation（60%）.So this method is suitable for the establishment a non-renal injury model of urinary stones.