泻肺平喘灵及其拆方对“肺合大肠”模型大鼠血清内毒素及肿瘤坏死因子-α的影响

Influence of Xiefei Pingchuanling and Its Decomposed Formulas on Serum Endotoxin and Tumor Necrosis Factor-α in Rat Model with ‘Interior and Exterior Relationship between Lung and Large Intestine’

目的:观察泻肺平喘灵及其拆方对"肺合大肠"模型大鼠血清内毒素(ET)和肿瘤坏死因子-α(TNF-α)变化的影响。方法:70只SD大鼠随机分为7组(正常组、模型组、解扎组、三拗组、三通组、三活组、泻肺平喘灵组),每组10只。采用直肠体外不全结扎法制备"肺合大肠"动物模型,造模3 d后处死模型组;三拗组以三拗汤3.92 g.kg-1 ig;三通组以三通方7.11 g.kg-1 ig;三活组以三活方10.54 g.kg-1 ig;泻肺平喘灵组以泻肺平喘灵16.66 g.kg-1 ig,正常组和解扎组以10 mL.kg-1生理盐水ig,3d后处死大鼠。观察大鼠血清ET和TNF-α的改变,观察泻肺平喘灵及其拆方的干预效果。结果:模型组大鼠造模后血清ET和TNF-α水平均明显升高(P<0.01),解扎组、泻肺平喘灵及其拆方组均降低,与模型组比较有非常显著性差异(P<0.01);泻肺平喘灵及其拆方组与解扎组比较也有显著性差异(P<0.01);部分药组之间呈协同的作用趋势。结论:泻肺平喘灵及其拆方能够降低模型大鼠血清ET和TNF-α水平,从而保护肺组织,减轻肺部病变,有效阻断内毒素血症对模型大鼠组织的损伤。

Objective：To observe the influence of Xiefei Pingchuanling and its decomposed formulas on serum changes of endotoxin（ET） and tumor necrosis factor-α（TNF-α） in model rats with ＇interior and exterior relationship between the lung and the large intestine＇.Method：Seventy SD rats were randomly divided into 7 groups： the normal group,the model group,the unhitch group,the Sanao group,the Santong group,the Sanhuo group and the Xiefei Pingchuanling group.The rat model of ＇interior and exterior relationship between the Lung and the Large intestine＇ was established to intestinal obstruction by incomplete ligation of the rectum in vitro.The rats in model group were killed after 3 days.The Sanao group was treated by Sanao decoction with the crude drug dosage of 3.92 g·kg-1 intragastrically,the Santong group was treated by Santong decoction with the crude drug dosage 7.11 g·kg-1 intragastrically,the Sanhuo group was teated by Sanhuo decoction with the crude drug dosage 10.54 g·kg-1 intragastrically,the Xiefei Pingchuanling group was treated by Xiefei Pingchuanling decoction with the crude drug dosage 16.66 g·kg-1 intragastrically.The rats were killed after 3 days of ig.The changes in serum ET and TNF-α were observed to investigate the interventional effect of Xiefei Pingchuanling and Its decomposed formulas.Result： The serum levels of ET and TNF-α were increased significantly in the model group（P0.01）.The serum levels of ET and TNF-α were decreased in the other groups compared with the models（P0.01）.The synergies existed in some groups.Conclusion：Xiefei Pingchuanling and its decomposed formulas can protect the lung tissue,alleviate pathological change and prevent the tissue of the model rats from endotoxemia.