糖脂平对2型糖尿病大鼠糖、脂毒性及胰腺组织氧化应激的影响

Effects of Tangzhiping on Glucose-toxicity,Lipotoxicity and Oxidative Stress of Pancreatic Tissue in Type 2 Diabetic Rats

目的:观察糖脂平对2型糖尿病大鼠糖、脂毒性及胰腺组织氧化应激的影响。方法:采用高脂喂养联合低剂量(30 mg.kg-1)链脲佐菌素诱导2型糖尿病大鼠模型。将糖尿病模型鼠随机分为模型组、糖脂平组、α-硫辛酸组,正常鼠为正常对照组,每组10只。糖脂平组予糖脂平按生药20 g.kg-1.d-1ig,α-硫辛酸组予20 mg.kg-1.d-1ig,其他2组大鼠予等体积蒸馏水ig,连续处理8周。观察体质量(BM)、空腹血糖(FPG)、糖化血红蛋白(HbA1c)、空腹胰岛素水平(FINS)、血清游离脂肪酸(FFA)以及胰腺组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平的变化;观察胰腺组织病理改变。结果:与模型组比较,经糖脂平、α-硫辛酸处理后两组大鼠体质量、血糖、空腹胰岛素、游离脂肪酸及氧化应激指标均明显改善(P<0.01或0.05);病理观察显示胰岛数量明显增加,胰岛组织结构部分修复。糖脂平组大鼠在FPG,HbA1c,FFA,FINS水平的改善方面优于α-硫辛酸组,病理改变亦相对较轻。结论:中药糖脂平能有效抑制2型糖尿病大鼠糖、脂毒性及胰腺组织的氧化应激损伤,保护胰岛细胞,改善胰岛素分泌及糖、脂代谢。

Objective：To observe the effects of Tangzhiping on the glucose-toxicity,lipotoxicity and the oxidative stress of pancreatic tissue in type 2 diabetic rats.Method： Type 2 diabetes mellitus was induced in male SD rats by feeding with high fat diet combined with intraperitoneal injection of low-dose streptozotocin（30 mg·kg-1）.Forty diabetic rats were randomly assigned to the model,Tangzhiping,alpha lipoic acid（ALA） and normal control groups.Rats in Tangzhiping group were performed intragastric administration of 20 g·kg-1·d-1 crude drug of Tangzhiping,and rats in ALA group were administrated with 20 mg·kg-1·d-1 ALA,other two groups were administrated with distilled water by equal volume.All four groups had been treated for eight weeks.Body mass（BM）,fast plasma glucose（FPG）,glycolated hemoglobin（HbA1c）,serum free fatty acid（FFA） and fast insulin（FINS） of rats were measured after eight weeks.And meanwhile superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,malonaldehyde（MDA） of pancreatic tissue were measured.Pathologic changes of pancreatic tissue were observed.Result： Compared with the model group,the levels of BM,FPG,HbA1c,FFA,FINS,SOD,GSH-Px and MDA of rats in both Tangzhiping group and ALA group were all significantly improved（P0.05 or 0.01） and pathological observation showed that the number of pancreas islet increased significantly and the injured pancreas were partially repaired in both groups.The effects of Tangzhiping group on BM,FPG,HbA1c,FFA,FINS were superior to those of ALA group,the differences were all significant（P0.01）.Conclusion： Tangzhiping can protect effectively the pancreatic islet β cells to improve insulin secretion and glucolipid metabolism by effectively inhibiting the glucose-toxicity,lipotoxicity and the oxidative stress of pancreatic tissue in type 2 diabetic rats.