益气通阳汤对血小板减少性紫癜小鼠模型的作用

Function of Yiqi Tongyang Decoction on Idiopathic Thrombocytopenic Purpura Animal Model

目的:观察益气通阳汤对特发性血小板减少性紫癜(ITP)模型小鼠外周血血小板(PLT)数、骨髓巨核细胞数和巨核细胞数分类、外周血T,B淋巴细胞亚群变化的影响。方法:设计用豚鼠抗小鼠血小板血清(APS)连续注射小鼠,造成ITP小鼠模型,分别给益气通阳汤(150,75,37.5 g.kg-1.d-1)组,正常对照组、模型对照组给予生理盐水,连续给药14 d。应用血细胞分析仪测定PLT、显微镜观察巨核细胞形态分类、塑料包埋法计数小鼠股骨巨核细胞数、流式细胞仪检测T,B淋巴细胞亚群。结果:模型小鼠外周血PLT数持续明显减低、骨髓巨核细胞数增多、其中原幼巨核细胞比例明显增高,产板巨核细胞比例明显减低,外周血CD3+CD4+T细胞减低,CD3+CD8+T细胞明显增高,CD4+/CD8+明显降低。给予益气通阳汤治疗小鼠的PLT明显增高、巨核细胞数减少、产板巨核细胞比例明显增高,CD3+CD4+T细胞增高,CD3+CD8+T细胞比例降低,CD4+/CD8+升高。结论:益气通阳汤能提升ITP模型小鼠PLT数,提高产板巨核细胞比例,改善T淋巴细胞的失衡,达到治疗ITP的作用。

Objective：To observe the impact of patients in disease of idiopathic thrombocytopenic purpura（ITP） of platelet count,marrow megakaryocyte count and class,T lymphocyte subset in peripheral blood on treatment with Yiqi Tongyang decoction.Method： It is designed to use guinea pig anti-mouse serum（APS） to inject to the mouse in order to induce the ITP mouse model.Then Yiqi Tongyang decoctions treatment group with 3 levels of greate,media and small is given.Separately to the treatement,the normal control group and model control group are also set,and all 3 group are maintained its treatment for continuel 14 days.The blood cell counter is used to detect platelet count,to observe megakaryocyte configuration by microscope,to count the megakaryocyte in mouse thighbone by plastic embedding and to detect T lymphocyte subset by cytometer.Result： In model mouse group,platelet,CD3＋CD4＋T cell are reduced significantely,and CD3＋CD8＋T cells are significantly increased.In Yiqi Tongyang mouse groups,platelet count increased significant,producing platelet megakaryocyte and CD4＋/CD8＋ were obviously increased.Megakaryocyte count reduced,CD3＋CD4＋T cell increased,and CD3＋CD8＋ T lymphocyte are significantly decreased,and CD4＋/CD8＋value is increased.Conclusion： Yiqi Tongyang decoction improves platelet count in ITP model mouse group,increase platelet producing megakaryocyte ratio,meliorate unbalance of T lymphocyte,that means the Yiqi Tongyang decoction is approved to be effective of treating ITP patients clearly in this study.