大蒜3个有效部位联合应用对人胃癌MKN45细胞的杀伤作用

Cytotoxic Effect of Combined Treatment of Three Cirsii Japonici Herba Components on Human Gastric Carcinoma MKN45 Cells

目的:观察大蒜有效部位及其组合物对人胃癌MKN45细胞杀伤的作用。方法:采用体外大鼠脾淋巴细胞增殖实验和MTT法测定细胞杀伤作用,筛选出具有免疫增强作用和细胞毒作用的大蒜有效部位进行组合,并对大蒜有效部位及其组合物对肿瘤细胞杀伤作用进行对比,运用正交试验筛选出大蒜3个有效部位联合应用对人胃癌MKN45细胞杀伤作用的最佳配比。结果:大蒜总多糖+大蒜总皂苷(2#+5#)对大鼠淋巴细胞增殖具有明显的促进作用,大蒜油、大蒜总多糖、大蒜总皂苷(1∶1∶1)的联合应用,其杀伤肿瘤细胞的作用(IC50 13.3μg.mL-1)明显优于同等剂量的2个有效部位组合及大蒜油的单独用药(IC50 35μg.mL-1)。对MKN45细胞杀伤作用的顺序为大蒜油>大蒜多糖>大蒜总皂苷,3个有效部位组合最佳配比为大蒜油∶大蒜多糖∶大蒜总皂苷1∶2∶1。结论:3个部位联合应用对肿瘤细胞的杀伤作用最强,并且组合物最大优势是在扶正、祛邪的同时,可能减少大蒜油的刺激性。

Objective：To investigate the cytotoxic effect of joint treatment of three Cirsii Japonici Herba components on human gastric carcinoma MKN45 cells.Method：MTT assay of rat lymphocyte and MKN45 cells proliferation in vitro were used to determine the effective components of Cirsii Japonici Herba.Orthogonal test were used to determine the optimum proportion of three components of Cirsii Japonici Herba on human gastric carcinoma MKN45 cells.Result： The combined treatment of Cirsii Japonici Herba polysaccharides and saponins（2#＋5#） significantly improved the lymphocyte proliferation of the cells.The cytotoxic activity of the combined treatment of Cirsii Japonici Herba oil,polysaccharides and saponins（1∶1∶1） to MKN45 cells（IC50 13.3 μg·mL-1） was obviously stronger than that of the combination of any two components or Cirsii Japonici Herba oil used alone（IC50 35 μg·mL-1）.The order of cytotoxic activity of three components of Cirsii Japonici Herba is oilpolysaccharidessaponins.The optimum proportion of three Cirsii Japonici Herba components on MKN45 cells was oil∶polysaccharides∶saponins 1∶2∶1.Conclusion： The combined treatment of three Cirsii Japonici Herba components had the strongest killing activity to human gastric carcinoma MKN45 cells.Except the effects of Fuzheng and Quxie come from the combined treatment,cytotoxicity and irritation of the Cirsii Japonici Herba oil were also decreased by the combined treatment.