糖肾清对DN模型大鼠肾组织形态学的影响

Morphologic Effect of Tangshenqing on Kidney of Rats with Diabetic Nephropathy

目的:探讨糖肾清对糖尿病大鼠肾脏形态学的影响。方法:将SD大鼠随机分为对照组、模型组、阳性药卡托普利(14 mg.kg-1)组、糖肾清高、中、低剂量(187,93.5,46.75 mg.kg-1)组,用STZ诱导大鼠糖尿病肾病(DN)模型,连续ig 12周后处死大鼠,取肾脏行HE,PAS染色,光镜下对肾组织病变进行分类评分,PAS染色下测定肾小球系膜基质相对面积。结果:糖肾清高剂量组的肾脏病变较模型组有所减轻,其肾小球基质相对面积较模型组明显减少(P<0.05)。结论:糖肾清能减轻DN大鼠肾小球细胞外基质的增生程度,对其肾脏病变具有保护作用。

Objective：To investigate morphologic effect of Tangshenqing on kidney of rats with diabetic nephropathy.Method：SD rats were induced diabetic nephropathy by STZ then randomly divided into normal control group,the model group,the positive group,the high dose of Tangshenqing group,the medium dose of Tangshenqing group and the low dose of the group.They were 187,93.5 and 46.75 mg·kg-1 respectively.After twelve weeks＇ oral drug administration,the rats were killed and the kidneys were harvested for hematoxylin and eosin and the periodic acid-sliver dyeing.The renal lesions were classified and scored under light microscope.The relative area of mesangium matrix was measured under the periodic acid-sliver dyeing.Result：The renal lesions of the high dose of Tangshenqing group was lightened than the model group.Mesangium matrix relative area of the high dose of Tangshenqing was obviously reduced than the model group（P0.05）.Conclusion：Tangshenqing may relive the extent of mesangium matrix hyperplasia for rats with diabetic nephropathy.