七味清咽喷雾剂防治流感的药理作用

Pharmacological Study on Qiwei Qingyan Aerosol for Treatment and Prevention of Influenza

目的:观察七味清咽喷雾剂对H1N1流感病毒的防治作用及与达菲联合应用的效果。方法:体外试验采用组织细胞培养法,观察不同浓度药物对流感病毒FM1病毒株致细胞病变(CPE)的作用;体内试验采用甲型H1N1流感病毒FM1株滴鼻感染小鼠造成肺炎模型,观察药物的疗效;达菲与七味清咽喷雾剂(剂量均降低50%)联合应用,观察中药的增效减毒作用。结果:①七味清咽喷雾剂对Hela和Hep-2细胞的最大无毒浓度(TC0)为0.192 g.L-1,该浓度有一定缓解流感病毒的致细胞病变作用,但作用较弱;②七味清咽喷雾剂高剂量经7 d连续给药,可明显降低H1N1流感病毒感染肺炎模型小鼠血清中TNF-α含量,提高IL-2水平;③七味清咽喷雾剂中剂量与达菲(较单独应用剂量降低50%)联合应用可降低感染小鼠肺脏指数,肺脏组织病变程度明显减轻;④二者联合应用降低感染动物血中神经氨酸酶(NA)活性的作用强于单用达菲组。结论:七味清咽喷雾剂通过影响小鼠免疫功能,改善流感病毒引起的小鼠肺炎症状,降低H1N1感染小鼠的肺指数;与达菲联合应用具有增效减毒作用。

Objective：To observe effect of Qiwei Qingyan aerosol for treatment and prevention and H1N1 virus as well as the efficacy when combining with tamiflu.Method： The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect（CPE） led by influenza virus FM1 with the aerosol of different concentrations in vitro.The swing influenza A（H1N1）virus FM1 was adopted to establish pneumonia model in mice by nasal dropping method to detect the efficacy of the aerosol for treatment in vivo.In addition,Combining the aerosol with tamiflu（both in 50% dose） to inspect the efficacy for efficiency improvement and toxicity depression of Chinese traditional medicine.Result： ①The maximal nontoxic concentration（TC0） of Qiwei Qingyan aerosol was 0.192 g·L-1 to Hela and Hep-2 cell.Within this concentration range it could relieve CPE induced by influenza virus,which was comparatively weak.②After 7-days successive high-dose medication of Qiwei Qingyan aerosol,the TNF-α level in serum of mice infected by H1N1 virus could be decreased apparently.Meanwhile,the IL-2 level could be increased significantly.③A combination of half dose tamiflu and moderate dose aerosol lowered the lung index of infected mice.As well,a lesser extent of lung tissue lesion was discovered 4.The ability of the combination to decrease the neuraminidase（NA） activity in the blood of the infected animals was superior to tamiflu solely used.Conclusion： By affecting the function of mice＇s immune system,Qiwei Qingyan aerosol improved the pneumonia symptoms induced by influenza virus and decreased the lung index of H1N1 infected mice.Furthermore,the combination with tamiflu possessed the effect of improving the efficiency and depressing the toxicity.