玉屏风散抑制小鼠Th2型变应性接触性皮炎

Yupingfeng San Inhibits Th2 Cell-mediated Allergic Contact Dermatitis in Mice

目的:应用异硫氰酸荧光素(FITC)诱导的Th2(Ⅱ型辅助性T细胞)型小鼠变应性接触性皮炎的模型,探讨玉屏风散对Th2型变应性接触性皮炎的作用。方法:小鼠在第1,2天用1.5%的FITC溶液致敏腹部皮肤2次,同时给予玉屏风散至第5天(诱导相给药),第6天用0.5%的FITC溶液攻击小鼠耳部皮肤,24 h后测定小鼠耳肿胀程度,并做耳病理组织学检查和耳匀浆中白介素-4(IL-4)测定。结果:玉屏风散6.5 g.kg-1(临床等效量)和13 g.kg-1诱导相给药均能显著抑制小鼠耳肿胀度,光镜下测量的耳厚度也表明玉屏风散明显抑制模型小鼠耳肿胀程度;病理组织学检查显示给予玉屏风散小鼠的耳炎症反应减轻,炎细胞浸润明显减少;玉屏风散6.5 g.kg-1组耳组织匀浆中IL-4水平较模型组显著降低。结论:玉屏风散诱导相给药对FITC诱导的小鼠Th2型变应性接触性皮炎有显著的抑制作用,作用机制可能与调节IL-4有关。

Objective：Yupingfeng San（YPFS） is a traditional formula used for allergy such as asthma,allergic rhinitis,and urticaria.Mice Th2 cell-mediated allergic contact dermatitis was utilized in this study to explore the effect of YPFS on allergic inflammatory.Method： The mice were topically sensitized on the shaved abdomens with 1.5%FITC solution on abdominal skin in the day 1 and day 2 and elicited on the right ear with 0.5%FITC solution at day 6.Mice were treated with either YPFS or normal saline from day 1 to day 5（induction phase）.Ear thickness was measured using a calibrated thickness gauge 24 h after the elicitation.Ear pathohistological examination was carried out by HE staining and IL-4 level of ear tissue was detected by ELISA.Result： The ear thickness,measured either by calibrated thickness gauge or under the microscope,was inhibited significantly by administration with YPFS 6.5 g·kg-1 and YPFS 13 g·kg-1 in induction phase.The pathohistological examination showed that the ear inflammation and infiltration of inflammatory cells was significantly reduced by YPFS.The levels of IL-4 in ear tissue were significantly lower in YPFS 6.5 g·kg-1 group compared with that in model group.Conclusion： Administration with YPFS in induction phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly,and the mechanism may be related with regulating IL-4.