川芎-白芷药对不同配比不同剂型对偏头痛动物模型的影响

Effect of Different Proportion and Different Dosage Forms of Combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix on Migraine in Animal Models

目的:观察川芎-白芷药对不同配比、不同剂型对偏头痛动物模型的影响。方法:利用硝酸甘油(10 mg.kg-1)ip一次造成大鼠偏头痛模型,sc利血平(0.4 mg.kg-1)7 d造成小鼠偏头痛模型,川芎-白芷药对3.5 g.kg-1.d-1进行治疗,对模型动物脑组织中5-羟色胺(5-HT)、5-羟基吲哚乙酸(5-HIAA)、降钙素基因相关肽(CGRP)含量进行检测。结果:偏头痛大鼠模型组脑组织中5-HT含量(720.44±92.1)ng.g-1,CGRP含量(618.32±139.3)pg.g-1;与模型组比较,川芎-白芷药对不同配比、不同剂型3.5 g.kg-1.d-1可升高偏头痛大鼠脑组织中5-HT 48.41%～73.18%,降低头痛大鼠脑组织中CGRP 38.02%～50.02%(P<0.01);与小鼠偏头痛模型组比较,川芎-白芷药对不同配比、不同剂型5 g.kg-1.d-1可升高偏头痛小鼠脑组织中5-HT 36.60%～69.52%(P<0.05,P<0.01)。结论:川芎-白芷药对不同配比、不同剂型主要是通过影响单胺类递质的释放,缓解神经源性炎症而改善偏头痛的症状。

Objective：To observe the effects of different proportion and different dosage forms of the combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix on migraine in animal models.Method：One experimental migraine animal model was duplicated by abdominal injection of nitroglycerin（10 mg·kg-1） once,the other was duplicated by subcutaneous injection of reserpine（0.4 mg·kg-1） seven days.After model establishment,the animals were treated with the combination of Chuanxiong Rhizoma and Radix Angelicae Dahuricae,and the levels of 5-hydroxytryptamine（5-HT）,5-hydroxyindole acedic acid（5-HIAA） and calatonin gene related peptide（CGRP） were determined.Result： Compared with model group,the combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix（3.5 g·kg-1·d-1）could raise the contents of 5-HT about 48.41%-73.18%,reduce the contents of CGRP about 38.02%-50.02%（P0.01）in migraine rats,and the combination（5 g·kg-1·d-1） could raise the contents of 5-HT about 36.60%-69.52% in migraine mouse（P0.05,P0.01）.Conclusion： The combination of Chuanxiong Rhizoma and Angelicae Dahuricae Radix has the best effect when it was given by the form of powders.It could improve migraine symptoms by controlling single of neurotransmitter dopamine release and alleviating neurogenic inflammation.